Tailoring Nanoparticle-Biofilm Interactions to Increase the Efficacy of Antimicrobial Agents Against Staphylococcus aureus

Files in This Item:
 File SizeFormat
Downloadijn-256227-tailoring-nanoparticle-biofilm-interactions-to-increase-the-.pdf3.71 MBAdobe PDF
Title: Tailoring Nanoparticle-Biofilm Interactions to Increase the Efficacy of Antimicrobial Agents Against Staphylococcus aureus
Authors: Fulaz, StephanieDevlin, HenryVitale, StefaniaQuinn, LauraO'Gara, James P.Casey, Eoin
Permanent link: http://hdl.handle.net/10197/12312
Date: 7-Jul-2020
Online since: 2021-07-01T16:05:14Z
Abstract: Background: Considering the timeline required for the development of novel antimicrobial drugs, increased attention should be given to repurposing old drugs and improving anti-microbial efficacy, particularly for chronic infections associated with biofilms. Methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA) are common causes of biofilm-associated infections but produce different biofilm matrices.MSSA biofilm cells are typically embedded in an extracellular polysaccharide matrix, whereas MRSA biofilms comprise predominantly of surface proteins and extracellular DNA (eDNA). Nanoparticles (NPs) have the potential to enhance the delivery of antimicro-bial agents into biofilms. However, the mechanisms which influence the interactions between NPs and the biofilm matrix are not yet fully understood. Methods:To investigate the influence of NPs surface chemistry on vancomycin (VAN) encapsulation and NP entrapment in MRSA and MSSA biofilms, mesoporous silica nano-particles (MSNs) with different surface functionalization (bare-B, amine-D, carboxyl-C,aromatic-A) were synthesised using an adapted Stöber method. The antibacterial efficacy of VAN-loaded MSNs was assessed against MRSA and MSSA biofilms. Results: The two negatively charged MSNs (MSN-B and MSN-C) showed a higher VAN loading in comparison to the positively charged MSNs (MSN-D and MSN-A). Cellular binding with MSN suspensions (0.25 mg mL−1) correlated with the reduced viability of both MSSA andMRSA biofilm cells. This allowed the administration of low MSNs concentrations while maintaining a high local concentration of the antibiotic surrounding the bacterial cells. Conclusion: Our data suggest that by tailoring the surface functionalization of MSNs,enhanced bacterial cell targeting can be achieved, leading to a novel treatment strategy for biofilm infections.
Funding Details: Science Foundation Ireland
Type of material: Journal Article
Publisher: Dove Medical Press
Journal: International Journal of Nanomedicine
Volume: 2020
Issue: 15
Start page: 4779
End page: 4791
Copyright (published version): 2020 the Authors
Keywords: Staphylococcus aureusMesporous silica nanparticlesEPS matrixAntimicrobialVancomycinNanoparticle-biofilm interactions
DOI: 10.2147/ijn.s256227
Language: en
Status of Item: Peer reviewed
ISSN: 1176-9114
This item is made available under a Creative Commons License: https://creativecommons.org/licenses/by-nc-nd/3.0/ie/
Appears in Collections:Chemical and Bioprocess Engineering Research Collection

Show full item record

Page view(s)

162
Last Week
4
Last month
checked on Jul 31, 2021

Download(s)

15
checked on Jul 31, 2021

Google ScholarTM

Check

Altmetric


If you are a publisher or author and have copyright concerns for any item, please email research.repository@ucd.ie and the item will be withdrawn immediately. The author or person responsible for depositing the article will be contacted within one business day.