Analysis of Protein Phosphorylation Using Phos-Tag Gels

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Title: Analysis of Protein Phosphorylation Using Phos-Tag Gels
Authors: Nagy, ZoltanComer, ShaneSmolenski, Albert P.
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Date: 25-Jul-2018
Online since: 2021-11-10T10:42:46Z
Abstract: Phos-tag gels are recent tools to dissect protein phosphorylation that operate by inducing a shift in the electrophoretic mobility of phosphorylated proteins compared to their nonphosphorylated counterparts. This article describes the preparation and electrophoresis of Zn2+ -Phos-tag gels along with electrotransfer of the separated phospho- and nonphosphoproteins onto a PVDF membrane using either wet-tank or semidry transfer. We also discuss the theory behind the technology with critical parameters to keep in mind for its successful application.
Funding Details: Science Foundation Ireland
Funding Details: British Heart Foundation
Type of material: Journal Article
Publisher: Wiley
Journal: Current Protocols in Protein Science
Volume: 93
Issue: 1
Copyright (published version): 2018 Wiley
Keywords: ZincPyridinesPhosphoproteinsphosphate‐affinity electrophoresisPhosphorylation
DOI: 10.1002/cpps.64
Language: en
Status of Item: Peer reviewed
ISSN: 1934-3655
This item is made available under a Creative Commons License:
Appears in Collections:Conway Institute Research Collection
Medicine Research Collection

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