Analysis of Protein Phosphorylation Using Phos-Tag Gels

Files in This Item:
 File SizeFormat
DownloadPhos-tag_6_final-acr.pdf776.24 kBAdobe PDF
Title: Analysis of Protein Phosphorylation Using Phos-Tag Gels
Authors: Nagy, ZoltanComer, ShaneSmolenski, Albert P.
Permanent link: http://hdl.handle.net/10197/12617
Date: 25-Jul-2018
Online since: 2021-11-10T10:42:46Z
Abstract: Phos-tag gels are recent tools to dissect protein phosphorylation that operate by inducing a shift in the electrophoretic mobility of phosphorylated proteins compared to their nonphosphorylated counterparts. This article describes the preparation and electrophoresis of Zn2+ -Phos-tag gels along with electrotransfer of the separated phospho- and nonphosphoproteins onto a PVDF membrane using either wet-tank or semidry transfer. We also discuss the theory behind the technology with critical parameters to keep in mind for its successful application.
Funding Details: Science Foundation Ireland
Funding Details: British Heart Foundation
Type of material: Journal Article
Publisher: Wiley
Journal: Current Protocols in Protein Science
Volume: 93
Issue: 1
Copyright (published version): 2018 Wiley
Keywords: ZincPyridinesPhosphoproteinsphosphate‐affinity electrophoresisPhosphorylation
DOI: 10.1002/cpps.64
Language: en
Status of Item: Peer reviewed
ISSN: 1934-3655
This item is made available under a Creative Commons License: https://creativecommons.org/licenses/by-nc-nd/3.0/ie/
Appears in Collections:Conway Institute Research Collection
Medicine Research Collection

Show full item record

Page view(s)

216
Last Week
18
Last month
checked on Nov 30, 2021

Download(s)

9
checked on Nov 30, 2021

Google ScholarTM

Check

Altmetric


If you are a publisher or author and have copyright concerns for any item, please email research.repository@ucd.ie and the item will be withdrawn immediately. The author or person responsible for depositing the article will be contacted within one business day.