Analysis of Protein Phosphorylation Using Phos-Tag Gels

DC FieldValueLanguage
dc.contributor.authorNagy, Zoltan-
dc.contributor.authorComer, Shane-
dc.contributor.authorSmolenski, Albert P.-
dc.date.accessioned2021-11-10T10:42:46Z-
dc.date.available2021-11-10T10:42:46Z-
dc.date.copyright2018 Wileyen_US
dc.date.issued2018-07-25-
dc.identifier.citationCurrent Protocols in Protein Scienceen_US
dc.identifier.issn1934-3655-
dc.identifier.urihttp://hdl.handle.net/10197/12617-
dc.description.abstractPhos-tag gels are recent tools to dissect protein phosphorylation that operate by inducing a shift in the electrophoretic mobility of phosphorylated proteins compared to their nonphosphorylated counterparts. This article describes the preparation and electrophoresis of Zn2+ -Phos-tag gels along with electrotransfer of the separated phospho- and nonphosphoproteins onto a PVDF membrane using either wet-tank or semidry transfer. We also discuss the theory behind the technology with critical parameters to keep in mind for its successful application.en_US
dc.description.sponsorshipScience Foundation Irelanden_US
dc.format.mediumPrint-Electronic-
dc.language.isoenen_US
dc.publisherWileyen_US
dc.rightsThis is the peer reviewed version of the following article: Nagy, Z., Comer, S., & Smolenski, A. (2018). Analysis of protein phosphorylation using Phos‐tag gels. Current Protocols in Protein Science, 93, e64. which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/cpps.64en_US
dc.subjectZincen_US
dc.subjectPyridinesen_US
dc.subjectPhosphoproteinsen_US
dc.subjectphosphate‐affinity electrophoresisen_US
dc.subjectPhosphorylationen_US
dc.titleAnalysis of Protein Phosphorylation Using Phos-Tag Gelsen_US
dc.typeJournal Articleen_US
dc.internal.authorcontactotheralbert.smolenski@ucd.ieen_US
dc.statusPeer revieweden_US
dc.identifier.volume93en_US
dc.identifier.issue1en_US
dc.citation.otherArticle Number: e64en_US
dc.identifier.doi10.1002/cpps.64-
dc.neeo.contributorNagy|Zoltan|aut|-
dc.neeo.contributorComer|Shane|aut|-
dc.neeo.contributorSmolenski|Albert P.|aut|-
dc.description.othersponsorshipBritish Heart Foundationen_US
dc.description.admin2021-04-13 JG: PDF replaced with correct version.en_US
dc.date.updated2020-09-18T14:52:22Z-
dc.identifier.grantid11/TIDA/B2021-
dc.identifier.grantidRG/15/13/31673-
dc.rights.licensehttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/en_US
item.fulltextWith Fulltext-
item.grantfulltextopen-
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Medicine Research Collection
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