Cold Atmospheric Plasma Stimulates Clathrin-Dependent Endocytosis to Repair Oxidised Membrane and Enhance Uptake of Nanomaterial in Glioblastoma Multiforme Cells

Files in This Item:
 File SizeFormat
DownloadCold_Atmospheric_Plasma_Stimulates_Clathrin-Depend.pdf5.39 MBAdobe PDF
Title: Cold Atmospheric Plasma Stimulates Clathrin-Dependent Endocytosis to Repair Oxidised Membrane and Enhance Uptake of Nanomaterial in Glioblastoma Multiforme Cells
Authors: He, ZhongleiLiu, KangzeScally, LaurenceBourke, Paulaet al.
Permanent link: http://hdl.handle.net/10197/12620
Date: 24-Apr-2020
Online since: 2021-11-10T11:13:44Z
Abstract: Cold atmospheric plasma (CAP) enhances uptake and accumulation of nanoparticles and promotes synergistic cytotoxicity against cancer cells. However, the mechanisms are not well understood. In this study, we investigate the enhanced uptake of theranostic nanomaterials by CAP. Numerical modelling of the uptake of gold nanoparticle into U373MG Glioblastoma multiforme (GBM) cells predicts that CAP may introduce a new uptake route. We demonstrate that cell membrane repair pathways play the main role in this stimulated new uptake route, following non-toxic doses of dielectric barrier discharge CAP. CAP treatment induces cellular membrane damage, mainly via lipid peroxidation as a result of reactive oxygen species (ROS) generation. Membranes rich in peroxidised lipids are then trafficked into cells via membrane repairing endocytosis. We confirm that the enhanced uptake of nanomaterials is clathrin-dependent using chemical inhibitors and silencing of gene expression. Therefore, CAP-stimulated membrane repair increases endocytosis and accelerates the uptake of gold nanoparticles into U373MG cells after CAP treatment. We demonstrate the utility of CAP to model membrane oxidative damage in cells and characterise a previously unreported mechanism of membrane repair to trigger nanomaterial uptake. This knowledge will underpin the development of new delivery strategies for theranostic nanoparticles into cancer cells.
Funding Details: Science Foundation Ireland
Funding Details: TU Dublin
Type of material: Journal Article
Publisher: Springer Nature
Journal: Scientific Reports
Volume: 10
Issue: 1
Copyright (published version): 2020 the Authors
Keywords: Lipid peroxidationCellular uptakeNeuronal degenerationMolecular mechanismsGold nanoparticlesOxidationToxicityCytotoxicityCholesterolMetabolism
DOI: 10.1038/s41598-020-63732-y
Language: en
Status of Item: Peer reviewed
ISSN: 2045-2322
This item is made available under a Creative Commons License: https://creativecommons.org/licenses/by/3.0/ie/
Appears in Collections:Biosystems and Food Engineering Research Collection

Show full item record

Page view(s)

201
Last Week
17
Last month
checked on Nov 30, 2021

Download(s)

9
checked on Nov 30, 2021

Google ScholarTM

Check

Altmetric


If you are a publisher or author and have copyright concerns for any item, please email research.repository@ucd.ie and the item will be withdrawn immediately. The author or person responsible for depositing the article will be contacted within one business day.