Cold Atmospheric Plasma Stimulates Clathrin-Dependent Endocytosis to Repair Oxidised Membrane and Enhance Uptake of Nanomaterial in Glioblastoma Multiforme Cells

DC FieldValueLanguage
dc.contributor.authorHe, Zhonglei-
dc.contributor.authorLiu, Kangze-
dc.contributor.authorScally, Laurence-
dc.contributor.authorBourke, Paula-
dc.contributor.authoret al.-
dc.date.accessioned2021-11-10T11:13:44Z-
dc.date.available2021-11-10T11:13:44Z-
dc.date.copyright2020 the Authorsen_US
dc.date.issued2020-04-24-
dc.identifier.citationScientific Reportsen_US
dc.identifier.issn2045-2322-
dc.identifier.urihttp://hdl.handle.net/10197/12620-
dc.description.abstractCold atmospheric plasma (CAP) enhances uptake and accumulation of nanoparticles and promotes synergistic cytotoxicity against cancer cells. However, the mechanisms are not well understood. In this study, we investigate the enhanced uptake of theranostic nanomaterials by CAP. Numerical modelling of the uptake of gold nanoparticle into U373MG Glioblastoma multiforme (GBM) cells predicts that CAP may introduce a new uptake route. We demonstrate that cell membrane repair pathways play the main role in this stimulated new uptake route, following non-toxic doses of dielectric barrier discharge CAP. CAP treatment induces cellular membrane damage, mainly via lipid peroxidation as a result of reactive oxygen species (ROS) generation. Membranes rich in peroxidised lipids are then trafficked into cells via membrane repairing endocytosis. We confirm that the enhanced uptake of nanomaterials is clathrin-dependent using chemical inhibitors and silencing of gene expression. Therefore, CAP-stimulated membrane repair increases endocytosis and accelerates the uptake of gold nanoparticles into U373MG cells after CAP treatment. We demonstrate the utility of CAP to model membrane oxidative damage in cells and characterise a previously unreported mechanism of membrane repair to trigger nanomaterial uptake. This knowledge will underpin the development of new delivery strategies for theranostic nanoparticles into cancer cells.en_US
dc.description.sponsorshipScience Foundation Irelanden_US
dc.format.mediumElectronic-
dc.language.isoenen_US
dc.publisherSpringer Natureen_US
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en_US
dc.subjectLipid peroxidationen_US
dc.subjectCellular uptakeen_US
dc.subjectNeuronal degenerationen_US
dc.subjectMolecular mechanismsen_US
dc.subjectGold nanoparticlesen_US
dc.subjectOxidationen_US
dc.subjectToxicityen_US
dc.subjectCytotoxicityen_US
dc.subjectCholesterolen_US
dc.subjectMetabolismen_US
dc.titleCold Atmospheric Plasma Stimulates Clathrin-Dependent Endocytosis to Repair Oxidised Membrane and Enhance Uptake of Nanomaterial in Glioblastoma Multiforme Cellsen_US
dc.typeJournal Articleen_US
dc.internal.authorcontactotherpaula.bourke@ucd.ieen_US
dc.statusPeer revieweden_US
dc.identifier.volume10en_US
dc.identifier.issue1en_US
dc.citation.otherArticle Number: 6985en_US
dc.identifier.doi10.1038/s41598-020-63732-y-
dc.neeo.contributorHe|Zhonglei|aut|-
dc.neeo.contributorLiu|Kangze|aut|-
dc.neeo.contributorScally|Laurence|aut|-
dc.neeo.contributorBourke|Paula|aut|-
dc.neeo.contributoret al.||aut|-
dc.description.othersponsorshipTU Dublinen_US
dc.date.updated2020-09-21T20:57:53Z-
dc.identifier.grantid14/IA/2626(T)-
dc.identifier.grantid14/IA/2626-
dc.identifier.grantid16/BBSRC/3391-
dc.rights.licensehttps://creativecommons.org/licenses/by/3.0/ie/en_US
item.fulltextWith Fulltext-
item.grantfulltextopen-
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