The Effect of Atmospheric Cold Plasma on Bacterial Stress Responses and Virulence Using Listeria monocytogenes Knockout Mutants

DC FieldValueLanguage
dc.contributor.authorPatange, Apurva-
dc.contributor.authorO'Byrne, Conor-
dc.contributor.authorBoehm, Daniela-
dc.contributor.authorBourke, Paula-
dc.contributor.authoret al.-
dc.date.accessioned2021-11-10T16:57:30Z-
dc.date.available2021-11-10T16:57:30Z-
dc.date.copyright2019 the Authorsen_US
dc.date.issued2019-12-11-
dc.identifier.citationFrontiers in Microbiologyen_US
dc.identifier.issn1664-302X-
dc.identifier.urihttp://hdl.handle.net/10197/12624-
dc.description.abstractListeria monocytogenes is an opportunistic intracellular pathogen commonly associated with serious infections and multiple food-borne outbreaks. In this study, we investigated the influence of atmospheric cold plasma (80 kV, 50 Hz) on L. monocytogenes (EGD-e) and its knockout mutants of sigB, rsbR, prfA, gadD, and lmo0799 genes at different treatment time intervals. Further, to ascertain if sub-lethal environmental stress conditions could influence L. monocytogenes survival and growth responses, atmospheric cold plasma (ACP) resistance was evaluated for the cultures exposed to cold (4°C) or acid (pH 4) stress for 1 h. The results demonstrate that both wild-type and knockout mutants were similarly affected after 1 min exposure to ACP (p > 0.05), with a difference in response noted only after 3 min of treatment. While all L. monocytogenes strains exposed to acid/cold stress were hypersensitive to ACP treatment and were significantly reduced or inactivated within 1 min of treatment (p < 0.05). The results indicate sigB and prfA are important for general stress resistance and biofilm, respectively, loss of these two genes significantly reduced bacterial resistance to ACP treatment. In addition, exposure to sub-lethal 1min ACP increased the gene expression of stress associated genes. SigB showed the highest gene expression, increasing by 15.60 fold, followed by gadD2 (7.19) and lmo0799 (8.6) after 1 min exposure. Overall, an increase in gene expression was seen in all stress associated genes analyzed both at 1 min treatment; while long treatment time reduced the gene expression and some cases down-regulated prfA and gadD3 gene expression. By comparing the response of mutants under ACP exposure to key processing parameters, the experimental results presented here provide a baseline for understanding the bacterial genetic response and resistance to cold plasma stress and offers promising insights for optimizing ACP applications.en_US
dc.description.sponsorshipDepartment of Agriculture, Food and the Marineen_US
dc.description.sponsorshipIrish Research Councilen_US
dc.format.mediumElectronic-eCollection-
dc.language.isoenen_US
dc.publisherFrontiers Mediaen_US
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.subjectListeria monocytogenesen_US
dc.subjectAtmospheric cold plasmaen_US
dc.subjectBiofilmen_US
dc.subjectGene expressionen_US
dc.subjectStress genesen_US
dc.subjectSub-lethal stressen_US
dc.titleThe Effect of Atmospheric Cold Plasma on Bacterial Stress Responses and Virulence Using Listeria monocytogenes Knockout Mutantsen_US
dc.typeJournal Articleen_US
dc.internal.authorcontactotherpaula.bourke@ucd.ieen_US
dc.statusPeer revieweden_US
dc.identifier.volume10en_US
dc.citation.otherArticle Number: 2841en_US
dc.identifier.doi10.3389/fmicb.2019.02841-
dc.neeo.contributorPatange|Apurva|aut|-
dc.neeo.contributorO'Byrne|Conor|aut|-
dc.neeo.contributorBoehm|Daniela|aut|-
dc.neeo.contributorBourke|Paula|aut|-
dc.neeo.contributoret al.||aut|-
dc.description.othersponsorshipFood Institutional Research Measure (FIRM)en_US
dc.date.updated2020-09-21T21:10:28Z-
dc.identifier.grantid13/F/444-
dc.rights.licensehttps://creativecommons.org/licenses/by/3.0/ie/en_US
item.grantfulltextopen-
item.fulltextWith Fulltext-
Appears in Collections:Biosystems and Food Engineering Research Collection
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