Cytotoxic and mutagenic potential of solutions exposed to cold atmospheric plasma

DC FieldValueLanguage
dc.contributor.authorBoehm, Daniela-
dc.contributor.authorHeslin, Caitlin-
dc.contributor.authorCullen, P. J.-
dc.contributor.authorBourke, Paula-
dc.date.accessioned2021-11-11T14:49:42Z-
dc.date.available2021-11-11T14:49:42Z-
dc.date.issued2016-02-24-
dc.identifier.citationScientific Reportsen_US
dc.identifier.issn2045-2322-
dc.identifier.urihttp://hdl.handle.net/10197/12635-
dc.description.abstractThe exposure of aqueous solutions to atmospheric plasmas results in the generation of relatively long-lived secondary products such as hydrogen peroxide which are biologically active and have demonstrated anti-microbial and cytotoxic activity. The use of plasma-activated solutions in applications such as microbial decontamination or anti-cancer treatments requires not only adequate performance on target cells but also a safe operating window regarding the impact on surrounding tissues. Furthermore the generation of plasma-activated fluids needs to be considered as a by-stander effect of subjecting tissue to plasma discharges. Cytotoxicity and mutagenicity assays using mammalian cell lines were used to elucidate the effects of solutions treated with di-electric barrier discharge atmospheric cold plasma. Plasma-treated PBS inhibited cell growth in a treatment time-dependent manner showing a linear correlation to the solutions' peroxide concentration which remained stable over several weeks. Plasma-treated foetal bovine serum (FBS) acting as a model for complex bio-fluids showed not only cytotoxic effects but also exhibited increased mutagenic potential as determined using the mammalian HPRT assay. Further studies are warranted to determine the nature, causes and effects of the cyto- and genotoxic potential of solutions exposed to plasma discharges to ensure long-term safety of novel plasma applications in medicine and healthcare.en_US
dc.description.sponsorshipDepartment of Agriculture, Food and the Marineen_US
dc.description.sponsorshipEuropean Commission - Seventh Framework Programme (FP7)en_US
dc.format.mediumElectronic-
dc.language.isoenen_US
dc.publisherSpringer Natureen_US
dc.subjectHela cellsen_US
dc.subjectCHO cellsen_US
dc.subjectCricetulusen_US
dc.subjectHydrogen peroxideen_US
dc.subjectSolutionsen_US
dc.subjectOxidantsen_US
dc.subjectMutagensen_US
dc.subjectInhibitory concentration 50en_US
dc.subjectCell adhesionen_US
dc.subjectCell proliferationen_US
dc.subjectCell survivalen_US
dc.subjectCricetinaeen_US
dc.subjectPlasma gasesen_US
dc.titleCytotoxic and mutagenic potential of solutions exposed to cold atmospheric plasmaen_US
dc.typeJournal Articleen_US
dc.internal.authorcontactotherpaula.bourke@ucd.ieen_US
dc.statusPeer revieweden_US
dc.identifier.volume6en_US
dc.identifier.issue1en_US
dc.citation.otherArtcile Number: 21464en_US
dc.identifier.doi10.1038/srep21464-
dc.neeo.contributorBoehm|Daniela|aut|-
dc.neeo.contributorHeslin|Caitlin|aut|-
dc.neeo.contributorCullen|P. J.|aut|-
dc.neeo.contributorBourke|Paula|aut|-
dc.date.updated2020-09-21T22:06:35Z-
dc.identifier.grantidFP7-SME-2013-
dc.identifier.grantid13/F/442-
dc.rights.licensehttps://creativecommons.org/licenses/by/3.0/ie/en_US
item.fulltextWith Fulltext-
item.grantfulltextopen-
Appears in Collections:Biosystems and Food Engineering Research Collection
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