Cold plasma inactivation of bacterial biofilms and reduction of quorum sensing regulated virulence factors

Title: Cold plasma inactivation of bacterial biofilms and reduction of quorum sensing regulated virulence factors
Authors: Ziuzina, DanaBoehm, DanielaPatil, SonalCullen, P. J.Bourke, Paula
Permanent link: http://hdl.handle.net/10197/12637
Date: 21-Sep-2015
Online since: 2021-11-11T14:56:05Z
Abstract: The main objectives of this work were to investigate the effect of atmospheric cold plasma (ACP) against a range of microbial biofilms commonly implicated in foodborne and healthcare associated human infections and against P. aeruginosa quorum sensing (QS)-regulated virulence factors, such as pyocyanin, elastase (Las B) and biofilm formation capacity post-ACP treatment. The effect of processing factors, namely treatment time and mode of plasma exposure on antimicrobial activity of ACP were also examined. Antibiofilm activity was assessed for E. coli, L. monocytogenes and S. aureus in terms of reduction of culturability and retention of metabolic activity using colony count and XTT assays, respectively. All samples were treated 'inpack' using sealed polypropylene containers with a high voltage dielectric barrier discharge ACP generated at 80 kV for 0, 60, 120 and 300 s and a post treatment storage time of 24 h. According to colony counts, ACP treatment for 60 s reduced populations of E. coli to undetectable levels, whereas 300 s was necessary to significantly reduce populations of L. monocytogenes and S. aureus biofilms. The results obtained from XTT assay indicated possible induction of viable but non culturable state of bacteria. With respect to P. aeruginosa QS-related virulence factors, the production of pyocyanin was significantly inhibited after short treatment times, but reduction of elastase was notable only after 300 s and no reduction in actual biofilm formation was achieved post-ACP treatment. Importantly, reduction of virulence factors was associated with reduction of the cytotoxic effects of the bacterial supernatant on CHO-K1 cells, regardless of mode and duration of treatment. The results of this study point to ACP technology as an effective strategy for inactivation of established biofilms and may play an important role in attenuation of virulence of pathogenic bacteria. Further investigation is warranted to propose direct evidence for the inhibition of QS and mechanisms by which this may occur.
Type of material: Journal Article
Publisher: PLoS
Journal: PLoS ONE
Volume: 10
Issue: 9
Copyright (published version): 2015 the Authors
Keywords: CHO cellsAnimalsCricetulusBacteriaBiofilmsVirulence factorsMicrosCricetinaeQuorum sensingCold temperaturePlasma gases
DOI: 10.1371/journal.pone.0138209
Language: en
Status of Item: Peer reviewed
ISSN: 1932-6203
This item is made available under a Creative Commons License: https://creativecommons.org/licenses/by/3.0/ie/
Appears in Collections:Biosystems and Food Engineering Research Collection

Show full item record

Page view(s)

176
Last Week
29
Last month
checked on Nov 30, 2021

Download(s)

7
checked on Nov 30, 2021

Google ScholarTM

Check

Altmetric


If you are a publisher or author and have copyright concerns for any item, please email research.repository@ucd.ie and the item will be withdrawn immediately. The author or person responsible for depositing the article will be contacted within one business day.