Cold plasma inactivation of bacterial biofilms and reduction of quorum sensing regulated virulence factors

DC FieldValueLanguage
dc.contributor.authorZiuzina, Dana-
dc.contributor.authorBoehm, Daniela-
dc.contributor.authorPatil, Sonal-
dc.contributor.authorCullen, P. J.-
dc.contributor.authorBourke, Paula-
dc.date.accessioned2021-11-11T14:56:05Z-
dc.date.available2021-11-11T14:56:05Z-
dc.date.copyright2015 the Authorsen_US
dc.date.issued2015-09-21-
dc.identifier.citationPLoS ONEen_US
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/10197/12637-
dc.description.abstractThe main objectives of this work were to investigate the effect of atmospheric cold plasma (ACP) against a range of microbial biofilms commonly implicated in foodborne and healthcare associated human infections and against P. aeruginosa quorum sensing (QS)-regulated virulence factors, such as pyocyanin, elastase (Las B) and biofilm formation capacity post-ACP treatment. The effect of processing factors, namely treatment time and mode of plasma exposure on antimicrobial activity of ACP were also examined. Antibiofilm activity was assessed for E. coli, L. monocytogenes and S. aureus in terms of reduction of culturability and retention of metabolic activity using colony count and XTT assays, respectively. All samples were treated 'inpack' using sealed polypropylene containers with a high voltage dielectric barrier discharge ACP generated at 80 kV for 0, 60, 120 and 300 s and a post treatment storage time of 24 h. According to colony counts, ACP treatment for 60 s reduced populations of E. coli to undetectable levels, whereas 300 s was necessary to significantly reduce populations of L. monocytogenes and S. aureus biofilms. The results obtained from XTT assay indicated possible induction of viable but non culturable state of bacteria. With respect to P. aeruginosa QS-related virulence factors, the production of pyocyanin was significantly inhibited after short treatment times, but reduction of elastase was notable only after 300 s and no reduction in actual biofilm formation was achieved post-ACP treatment. Importantly, reduction of virulence factors was associated with reduction of the cytotoxic effects of the bacterial supernatant on CHO-K1 cells, regardless of mode and duration of treatment. The results of this study point to ACP technology as an effective strategy for inactivation of established biofilms and may play an important role in attenuation of virulence of pathogenic bacteria. Further investigation is warranted to propose direct evidence for the inhibition of QS and mechanisms by which this may occur.en_US
dc.format.mediumElectronic-eCollection-
dc.language.isoenen_US
dc.publisherPLoSen_US
dc.subjectCHO cellsen_US
dc.subjectAnimalsen_US
dc.subjectCricetulusen_US
dc.subjectBacteriaen_US
dc.subjectBiofilmsen_US
dc.subjectVirulence factorsen_US
dc.subjectMicrosen_US
dc.subjectCricetinaeen_US
dc.subjectQuorum sensingen_US
dc.subjectCold temperatureen_US
dc.subjectPlasma gasesen_US
dc.titleCold plasma inactivation of bacterial biofilms and reduction of quorum sensing regulated virulence factorsen_US
dc.typeJournal Articleen_US
dc.internal.authorcontactotherpaula.bourke@ucd.ieen_US
dc.statusPeer revieweden_US
dc.identifier.volume10en_US
dc.identifier.issue9en_US
dc.citation.otherArticle Number: e0138209en_US
dc.identifier.doi10.1371/journal.pone.0138209-
dc.neeo.contributorZiuzina|Dana|aut|-
dc.neeo.contributorBoehm|Daniela|aut|-
dc.neeo.contributorPatil|Sonal|aut|-
dc.neeo.contributorCullen|P. J.|aut|-
dc.neeo.contributorBourke|Paula|aut|-
dc.date.updated2020-09-21T22:08:53Z-
dc.identifier.grantidFP7/2207-2013-
dc.identifier.grantid285820-
dc.rights.licensehttps://creativecommons.org/licenses/by/3.0/ie/en_US
item.fulltextWith Fulltext-
item.grantfulltextopen-
Appears in Collections:Biosystems and Food Engineering Research Collection
Show simple item record

Page view(s)

217
Last Week
4
Last month
32
checked on Jan 17, 2022

Download(s)

11
checked on Jan 17, 2022

Google ScholarTM

Check

Altmetric


If you are a publisher or author and have copyright concerns for any item, please email research.repository@ucd.ie and the item will be withdrawn immediately. The author or person responsible for depositing the article will be contacted within one business day.