Deposition of Cell Culture Coatings Using a Cold Plasma Deposition Method

Title: Deposition of Cell Culture Coatings Using a Cold Plasma Deposition Method
Authors: O'Sullivan, DenisMcArdle, HazelNg, Sing WeiBourke, Paula
Permanent link: http://hdl.handle.net/10197/12639
Date: 24-Sep-2020
Online since: 2021-11-11T15:01:25Z
Abstract: Collagen coatings were applied onto polystyrene microplates using a cold atmospheric pressure plasma process. The coatings were compared to standard wet chemical collagen thin films using microscopy, surface energy, infra-red spectroscopy, electrophoresis, and cell culture techniques. Thin films were also deposited on gold electrodes using both coating methods and their structural and barrier properties probed using cyclic voltammetry. While the wet chemical technique produced a thicker deposit, both films appear equivalent in terms of coverage, porosity, structure, and chemistry. Significantly, the cold plasma method preserves both the primary and secondary structure of the protein and this results in high biocompatibility and cell activity that is at least equivalent to the standard wet chemical technique. The significance of these results is discussed in relation to the benefits of a single step plasma coating in comparison to the traditional multi-step aseptic coating technique.
Funding Details: European Commission - European Regional Development Fund
Irish Research Council
Science Foundation Ireland
Funding Details: FutureNeuro
Type of material: Journal Article
Publisher: MDPI
Journal: Applied Sciences
Volume: 10
Issue: 19
Copyright (published version): 2020 the Authors
Keywords: Plasma polymerizationCorona dischargeBiomolecule depositionCollagenProtein voltammerty
DOI: 10.3390/app10196670
Language: en
Status of Item: Peer reviewed
This item is made available under a Creative Commons License: https://creativecommons.org/licenses/by/3.0/ie/
Appears in Collections:Biosystems and Food Engineering Research Collection

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