Deposition of Cell Culture Coatings Using a Cold Plasma Deposition Method

DC FieldValueLanguage
dc.contributor.authorO'Sullivan, Denis-
dc.contributor.authorMcArdle, Hazel-
dc.contributor.authorNg, Sing Wei-
dc.contributor.authorBourke, Paula-
dc.date.accessioned2021-11-11T15:01:25Z-
dc.date.available2021-11-11T15:01:25Z-
dc.date.copyright2020 the Authorsen_US
dc.date.issued2020-09-24-
dc.identifier.citationApplied Sciencesen_US
dc.identifier.urihttp://hdl.handle.net/10197/12639-
dc.description.abstractCollagen coatings were applied onto polystyrene microplates using a cold atmospheric pressure plasma process. The coatings were compared to standard wet chemical collagen thin films using microscopy, surface energy, infra-red spectroscopy, electrophoresis, and cell culture techniques. Thin films were also deposited on gold electrodes using both coating methods and their structural and barrier properties probed using cyclic voltammetry. While the wet chemical technique produced a thicker deposit, both films appear equivalent in terms of coverage, porosity, structure, and chemistry. Significantly, the cold plasma method preserves both the primary and secondary structure of the protein and this results in high biocompatibility and cell activity that is at least equivalent to the standard wet chemical technique. The significance of these results is discussed in relation to the benefits of a single step plasma coating in comparison to the traditional multi-step aseptic coating technique.en_US
dc.description.sponsorshipEuropean Commission - European Regional Development Funden_US
dc.description.sponsorshipIrish Research Councilen_US
dc.description.sponsorshipScience Foundation Irelanden_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly citeden_US
dc.subjectPlasma polymerizationen_US
dc.subjectCorona dischargeen_US
dc.subjectBiomolecule depositionen_US
dc.subjectCollagenen_US
dc.subjectProtein voltammertyen_US
dc.titleDeposition of Cell Culture Coatings Using a Cold Plasma Deposition Methoden_US
dc.typeJournal Articleen_US
dc.internal.authorcontactotherpaula.bourke@ucd.ieen_US
dc.statusPeer revieweden_US
dc.identifier.volume10en_US
dc.identifier.issue19en_US
dc.citation.otherArticle Number: 6670en_US
dc.identifier.doi10.3390/app10196670-
dc.neeo.contributorO'Sullivan|Denis|aut|-
dc.neeo.contributorMcArdle|Hazel|aut|-
dc.neeo.contributorNg|Sing Wei|aut|-
dc.neeo.contributorBourke|Paula|aut|-
dc.description.othersponsorshipFutureNeuroen_US
dc.date.updated2020-09-29T13:49:19Z-
dc.identifier.grantidEBPPG/2018/82-
dc.identifier.grantid16/RC/3948-
dc.identifier.grantid16/BBSRC/3391-
dc.identifier.grantidEBPPG/2018/82-
dc.rights.licensehttps://creativecommons.org/licenses/by/3.0/ie/en_US
item.fulltextWith Fulltext-
item.grantfulltextopen-
Appears in Collections:Biosystems and Food Engineering Research Collection
Show simple item record

Page view(s)

228
Last Week
4
Last month
31
checked on Jan 20, 2022

Download(s)

15
checked on Jan 20, 2022

Google ScholarTM

Check

Altmetric


If you are a publisher or author and have copyright concerns for any item, please email research.repository@ucd.ie and the item will be withdrawn immediately. The author or person responsible for depositing the article will be contacted within one business day.