Direct Plasma Deposition of Collagen on 96-Well Polystyrene Plates for Cell Culture

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Title: Direct Plasma Deposition of Collagen on 96-Well Polystyrene Plates for Cell Culture
Authors: O'Sullivan, DenisO'Neill, LiamBourke, Paula
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Date: 24-Sep-2020
Online since: 2021-11-11T15:04:33Z
Abstract: A cold atmospheric plasma unit was used to deposit a biologic, in this case collagen, onto a surface. A collagen coating was applied to 96-well polystyrene plates at a range of powers to determine the effects of the plasma power on the coating structure and viability. Plasma characterization was carried out using voltage, current, and power measurements. Coating characterization was completed using gravimetric measurement, cell growth, water contact angle, as well as spectroscopic analysis and compared to commercial collagen-coated plates. Cell culture studies were also undertaken. The plasma coating matched the performance of the commercial plate but dramatically reduced production time and cost. This method could allow for automated inline production of collagen-coated plates for cell culture applications.
Funding Details: Irish Research Council
Type of material: Journal Article
Publisher: ACS
Journal: ACS Omega
Volume: 5
Issue: 39
Start page: 25069
End page: 25076
Copyright (published version): 2020 American Chemical Society
Keywords: Coating materialsDepositionPlasmaPowerBiopolymers
DOI: 10.1021/acsomega.0c02073
Language: en
Status of Item: Peer reviewed
ISSN: 2470-1343
This item is made available under a Creative Commons License:
Appears in Collections:Biosystems and Food Engineering Research Collection

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