Biomolecules as Model Indicators of In Vitro and In Vivo Cold Plasma Safety

DC FieldValueLanguage
dc.contributor.authorHeslin, Caitlin-
dc.contributor.authorBoehm, Daniela-
dc.contributor.authorGilmore, Brendan F.-
dc.contributor.authorBourke, Paula-
dc.contributor.authoret al.-
dc.date.accessioned2021-11-11T15:26:15Z-
dc.date.available2021-11-11T15:26:15Z-
dc.date.copyright2021 the Authorsen_US
dc.date.issued2021-01-14-
dc.identifier.citationFrontiers in Physicsen_US
dc.identifier.issn2296-424X-
dc.identifier.urihttp://hdl.handle.net/10197/12645-
dc.description.abstractThe potential applications for cold plasma in medicine are extensive, from microbial inactivation and induction of apoptosis in cancer cells to stimulating wound healing and enhancing the blood coagulation cascade. The safe bio-medical application of cold plasma and subsequent effect on complex biological pathways requires precision and a distinct understanding of how physiological redox chemistry is manipulated. Chemical modification of biomolecules such as carbohydrates, proteins, and lipids treated with cold plasma have been characterized, however, the context of how alterations of these molecules affect cell behavior or in vivo functionality has not been determined. Thus, this study examines the cytotoxic and mutagenic effects of plasma-treated molecules in vitro using CHO-K1 cells and in vivo in Galleria mellonella larvae. Specifically, albumin, glucose, cholesterol, and arachidonic acid were chosen as representative biomolecules, with established involvement in diverse bioprocesses including; cellular respiration, intracellular transport, cell signaling or membrane structure. Long- and short-term effects depended strongly on the molecule type and the treatment milieu indicating the impact of chemical and physical modifications on downstream biological pathways. Importantly, absence of short-term toxicity did not always correlate with absence of longer-term effects, indicating the need to comprehensively assess ongoing effects for diverse biological applications.en_US
dc.description.sponsorshipIrish Research Councilen_US
dc.description.sponsorshipScience Foundation Irelanden_US
dc.language.isoenen_US
dc.publisherFrontiers Mediaen_US
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.subjectIn vivo toxicityen_US
dc.subjectAtmospheric-pressure plasmaen_US
dc.subjectArachidonic-aciden_US
dc.subjectCholesterol oxidationen_US
dc.subjectEscherichia-Colien_US
dc.subjectCytotoxicityen_US
dc.subjectInactivationen_US
dc.subjectInductionen_US
dc.subjectCellsen_US
dc.subjectARTPen_US
dc.titleBiomolecules as Model Indicators of In Vitro and In Vivo Cold Plasma Safetyen_US
dc.typeJournal Articleen_US
dc.internal.authorcontactotherpaula.bourke@ucd.ieen_US
dc.statusPeer revieweden_US
dc.identifier.volume8en_US
dc.citation.otherArticle Number: 613046en_US
dc.identifier.doi10.3389/fphy.2020.613046-
dc.neeo.contributorHeslin|Caitlin|aut|-
dc.neeo.contributorBoehm|Daniela|aut|-
dc.neeo.contributorGilmore|Brendan F.|aut|-
dc.neeo.contributorBourke|Paula|aut|-
dc.neeo.contributoret al.||aut|-
dc.date.updated2021-09-29T10:22:19Z-
dc.identifier.grantid14/IA/2626(T)-
dc.rights.licensehttps://creativecommons.org/licenses/by/3.0/ie/en_US
item.fulltextWith Fulltext-
item.grantfulltextopen-
Appears in Collections:Biosystems and Food Engineering Research Collection
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