A small secreted protein from Zymoseptoria tritici interacts with a wheat E3 ubiquitin to promote disease

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Title: A small secreted protein from Zymoseptoria tritici interacts with a wheat E3 ubiquitin to promote disease
Authors: Karki, Sujit JungReilly, AislingZhou, BinbinMascarello, MaurizioBurke, JamesDoohan, Fiona M.Douchkov, DimitarSchweizer, PatrickFeechan, Angela
Permanent link: http://hdl.handle.net/10197/12757
Date: 2-Feb-2021
Online since: 2022-02-10T12:34:42Z
Abstract: Septoria Tritici Blotch, caused by the ascomycete fungus Zymoseptoria tritici, is a major threat to wheat production worldwide. The Z. tritici genome encodes many small, secreted proteins (ZtSSP) that likely play a key role in the successful colonisation of host tissues. However, few of these ZtSSPs have been functionally characterised for their role during infection. In this study, we identified and characterised a small, conserved cysteine-rich secreted effector from Zymoseptoria tritici which has homologues in other plant pathogens in the dothideomycetes. ZtSSP2 was expressed throughout Z. tritici infection in wheat with the highest levels observed early during infection. A yeast two-hybrid assay revealed an interaction between ZtSSP2 and wheat E3 ubiquitin ligase in yeast and this was further confirmed in planta using bimolecular fluorescence complementation, and co-immunoprecipitation. Down-regulation of this wheat E3 ligase using virus-induced gene silencing, increased the susceptibility of wheat to Septoria tritici blotch (STB). Together these results suggest that TaE3UBQ likely plays a role in plant immunity to defend against Z. tritici.
Funding Details: Department of Agriculture, Food and the Marine
European Commission Horizon 2020
Science Foundation Ireland
Type of material: Journal Article
Publisher: Oxford University Press
Journal: Journal of Experimental Botany
Volume: 72
Issue: 2
Start page: 733
End page: 746
Copyright (published version): 2020 the Authors
Keywords: Zymoseptoria triticiFungal pathogenDiseaseWheatE3 ubiquitin ligaseEffector
DOI: 10.1093/jxb/eraa489
Language: en
Status of Item: Peer reviewed
ISSN: 0022-0957
This item is made available under a Creative Commons License: https://creativecommons.org/licenses/by/3.0/ie/
Appears in Collections:Biology & Environmental Science Research Collection
Earth Institute Research Collection
Agriculture and Food Science Research Collection

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