Covariation of Pluripotency Markers and Biomechanical Properties in Mouse Embryonic Stem Cells

Title: Covariation of Pluripotency Markers and Biomechanical Properties in Mouse Embryonic Stem Cells
Authors: Brookes, OliverThorpe, Stephen D.Rigby Evans, OlgaKeeling, Michael C.Lee, David A.
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Date: 16-May-2022
Online since: 2022-07-19T08:14:34Z
Abstract: Pluripotent cells are subject to much interest as a source of differentiated cellular material for research models, regenerative medical therapies and novel applications such as lab-cultured meat. Greater understanding of the pluripotent state and control over its differentiation is therefore desirable. The role of biomechanical properties in directing cell fate and cell behavior has been increasingly well described in recent years. However, many of the mechanisms which control cell morphology and mechanical properties in somatic cells are absent from pluripotent cells. We leveraged naturally occurring variation in biomechanical properties and expression of pluripotency genes in murine ESCs to investigate the relationship between these parameters. We observed considerable variation in a Rex1-GFP expression reporter line and found that this variation showed no apparent correlation to cell spreading morphology as determined by circularity, Feret ratio, phase contrast brightness or cell spread area, either on a parameter-by-parameter basis, or when evaluated using a combined metric derived by principal component analysis from the four individual criteria. We further confirmed that cell volume does not co-vary with Rex1-GFP expression. Interestingly, we did find that a subpopulation of cells that were readily detached by gentle agitation collectively exhibited higher expression of Nanog, and reduced LmnA expression, suggesting that elevated pluripotency gene expression may correlate with reduced adhesion to the substrate. Furthermore, atomic force microscopy and quantitative fluorescent imaging revealed a connection between cell stiffness and Rex1-GFP reporter expression. Cells expressing high levels of Rex1-GFP are consistently of a relatively low stiffness, while cells with low levels of Rex1-GFP tend toward higher stiffness values. These observations indicate some interaction between pluripotency gene expression and biomechanical properties, but also support a strong role for other interactions between the cell culture regime and cellular biomechanical properties, occurring independently of the core transcriptional network that supports pluripotency.
Funding Details: Engineering and Physical Sciences Research Council
Biotechnology and Biological Sciences Research Council
Type of material: Journal Article
Publisher: Frontiers Media
Journal: Frontiers in Cell and Developmental Biology
Volume: 10
Start page: 1
End page: 13
Copyright (published version): 2022 The Authors
Keywords: Mouse embryonic stem cellsPluripotency markerCell stiffnessCell adhesionCell morphology
DOI: 10.3389/fcell.2022.858884
Language: en
Status of Item: Peer reviewed
ISSN: 2296-634X
This item is made available under a Creative Commons License:
Appears in Collections:Conway Institute Research Collection
Medicine Research Collection

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