Advanced analytical strategies for the characterisation of biotherapeutics

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Title: Advanced analytical strategies for the characterisation of biotherapeutics
Authors: Jakes, Craig
Permanent link: http://hdl.handle.net/10197/13137
Date: 2022
Online since: 2022-09-28T11:54:48Z
Abstract: Monoclonal antibodies (mAbs) are the most dominant selling class of biotherapeutics in the global market. These complex biomolecules are produced through mammalian cell culture and are prone to structural heterogeniety. This heterogeniety can have an adverse effect on the overall stability and efficacy of the drug product and must be closely monitored through product characterisation. Characterisation of mAbs can be carried out on the intact, subunit and peptide level. The drawback with traditional characterisation techniques include high sample requirement, high level of expertise needed to produce reporducible data and the methods involved are usually time consuming. The objective of this work was develop characterisation strategies at all three levels using novel instrumentation to overcome these drawbacks and improve our understanding of product characteristics at all stages of the mAb production cycle.
Type of material: Doctoral Thesis
Publisher: University College Dublin. School of Chemical and Bioprocess Engineering
Qualification Name: Ph.D.
Copyright (published version): 2022 the Author
Keywords: Monoclonal antibodiesLiquid chromatographyMass spectrometryBiotechnology
Language: en
Status of Item: Peer reviewed
This item is made available under a Creative Commons License: https://creativecommons.org/licenses/by-nc-nd/3.0/ie/
Appears in Collections:Chemical and Bioprocess Engineering Theses

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