Rapid depletion of dissolved oxygen in 96 well microtitre plate Staphylococcus epidermidis biofilm assays promotes biofilm development and is influenced by inoculum cell concentration

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Title: Rapid depletion of dissolved oxygen in 96 well microtitre plate Staphylococcus epidermidis biofilm assays promotes biofilm development and is influenced by inoculum cell concentration
Authors: Cotter, John J.
O'Gara, James P.
Casey, Eoin
Permanent link: http://hdl.handle.net/10197/2729
Date: 1-Aug-2009
Abstract: Biofilm-related research using 96-well microtiter plates involves static incubation of plates indiscriminate of environmental conditions, making oxygen availability an important variable which has not been considered to date. By directly measuring dissolved oxygen concentration over time we report here that dissolved oxygen is rapidly consumed in Staphylococcus epidermidis biofilm cultures grown in 96-well plates irrespective of the oxygen concentration in the gaseous environment in which the plates are incubated. These data indicate that depletion of dissolved oxygen during growth of bacterial biofilm cultures in 96-well plates may significantly influence biofilm production. Furthermore higher inoculum cell concentrations are associated with more rapid consumption of dissolved oxygen and higher levels of S. epidermidis biofilm production. Our data reveal that oxygen depletion during bacterial growth in 96-well plates may significantly influence biofilm production and should be considered in the interpretation of experimental data using this biofilm model.
Funding Details: Science Foundation Ireland
Type of material: Journal Article
Publisher: Wiley
Copyright (published version): 2009 Wiley Periodicals, Inc.
Keywords: Biofilm;Oxygen;Staphylococcus epidermidis;Microtitre;96-well plate
Subject LCSH: Biofilms
Oxygen
Staphylococcus
Microplates
DOI: 10.1002/bit.22335
Language: en
Status of Item: Peer reviewed
Appears in Collections:Conway Institute Research Collection
Chemical and Bioprocess Engineering Research Collection
CSCB Research Collection

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