Rapid depletion of dissolved oxygen in 96 well microtitre plate Staphylococcus epidermidis biofilm assays promotes biofilm development and is influenced by inoculum cell concentration

Files in This Item:
File Description SizeFormat 
2009-cotter-96-well-BB.pdf139.4 kBAdobe PDFDownload
Title: Rapid depletion of dissolved oxygen in 96 well microtitre plate Staphylococcus epidermidis biofilm assays promotes biofilm development and is influenced by inoculum cell concentration
Authors: Cotter, John J.O'Gara, James P.Casey, Eoin
Permanent link: http://hdl.handle.net/10197/2729
Date: 1-Aug-2009
Online since: 2011-01-19T14:27:44Z
Abstract: Biofilm-related research using 96-well microtiter plates involves static incubation of plates indiscriminate of environmental conditions, making oxygen availability an important variable which has not been considered to date. By directly measuring dissolved oxygen concentration over time we report here that dissolved oxygen is rapidly consumed in Staphylococcus epidermidis biofilm cultures grown in 96-well plates irrespective of the oxygen concentration in the gaseous environment in which the plates are incubated. These data indicate that depletion of dissolved oxygen during growth of bacterial biofilm cultures in 96-well plates may significantly influence biofilm production. Furthermore higher inoculum cell concentrations are associated with more rapid consumption of dissolved oxygen and higher levels of S. epidermidis biofilm production. Our data reveal that oxygen depletion during bacterial growth in 96-well plates may significantly influence biofilm production and should be considered in the interpretation of experimental data using this biofilm model.
Funding Details: Science Foundation Ireland
Type of material: Journal Article
Publisher: Wiley
Journal: Biotechnology and Bioengineering
Volume: 103
Issue: 5
Start page: 1042
End page: 1047
Copyright (published version): 2009 Wiley Periodicals, Inc.
Keywords: BiofilmOxygenStaphylococcus epidermidisMicrotitre96-well plate
Subject LCSH: Biofilms
Oxygen
Staphylococcus
Microplates
DOI: 10.1002/bit.22335
Other versions: http://dx.doi.org/10.1002/bit.22335
Language: en
Status of Item: Peer reviewed
Appears in Collections:Conway Institute Research Collection
Chemical and Bioprocess Engineering Research Collection
CSCB Research Collection

Show full item record

SCOPUSTM   
Citations 20

13
Last Week
0
Last month
checked on Mar 30, 2020

Page view(s) 20

1,489
Last Week
4
Last month
checked on Apr 6, 2020

Download(s) 20

740
checked on Apr 6, 2020

Google ScholarTM

Check

Altmetric


This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. For other possible restrictions on use please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.