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Rapid depletion of dissolved oxygen in 96 well microtitre plate Staphylococcus epidermidis biofilm assays promotes biofilm development and is influenced by inoculum cell concentration
Author(s)
Date Issued
2009-08-01
Date Available
2011-01-19T14:27:44Z
Abstract
Biofilm-related research using 96-well microtiter plates involves static incubation of plates indiscriminate of environmental conditions, making oxygen availability an important variable which has not been considered to date. By directly measuring dissolved oxygen concentration over time we report here that dissolved oxygen is rapidly consumed in Staphylococcus epidermidis biofilm cultures grown in 96-well plates irrespective of the oxygen concentration in the gaseous environment in which the plates are incubated. These data indicate that depletion of dissolved oxygen during growth of bacterial biofilm cultures in 96-well plates may significantly influence biofilm production. Furthermore higher inoculum cell concentrations are associated with more rapid consumption of dissolved oxygen and higher levels of S. epidermidis biofilm production. Our data reveal that oxygen depletion during bacterial growth in 96-well plates may significantly influence biofilm production and should be considered in the interpretation of experimental data using this biofilm model.
Sponsorship
Science Foundation Ireland
Type of Material
Journal Article
Publisher
Wiley
Journal
Biotechnology and Bioengineering
Volume
103
Issue
5
Start Page
1042
End Page
1047
Copyright (Published Version)
2009 Wiley Periodicals, Inc.
Subject – LCSH
Biofilms
Oxygen
Staphylococcus
Microplates
Web versions
Language
English
Status of Item
Peer reviewed
ISSN
1097-0290
This item is made available under a Creative Commons License
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