Antibacterial effects of poly(2-(dimethylamino ethyl)methacrylate) against selected Gram-positive and Gram-negative bacteria

Files in This Item:
 File SizeFormat
DownloadBiomacromolecules 12102009 MASTER.pdf478.43 kBAdobe PDF
Title: Antibacterial effects of poly(2-(dimethylamino ethyl)methacrylate) against selected Gram-positive and Gram-negative bacteria
Authors: Rawlinson, Lee-Anne BettyRyan, Sinéad M.Mantovani, GiuseppeSyrett, Jay A.Haddleton, David M.Brayden, David James
Permanent link:
Date: 8-Feb-2010
Online since: 2011-02-15T11:43:47Z
Abstract: Antimicrobial coatings can reduce the occurrence of medical device-related bacterial infections. Poly(2-(dimethylamino ethyl)methacrylate)) (pDMAEMA) is one such polymer that is being researched in this regard. The aims of this study were to (1) elucidate pDMAEMA’s antimicrobial activity against a range of Gram-positive and Gram-negative bacteria and (2) to investigate its antimicrobial mode of action. The methods used include determination of minimum inhibitory concentration (MIC) values against various bacteria and the effect of pH and temperature on antimicrobial activity. The ability of pDMAEMA to permeabilise bacterial membranes was determined using the dyes 1-N-phenyl-naphthylamine (NPN) and Calcein-AM. Flow cytometry was used to investigate pDMAEMA’s capacity to be internalised by bacteria and to determine effects on bacterial cell cycling. pDMAEMA was bacteriostatic against Gram-negative bacteria with MIC values between 0.1–10 mg/ml. MIC values against Gram-positive bacteria were variable. pDMAEMA was active against Gram-positive bacteria around its pKa and at lower pH values, while it was active against Gram-negative bacteria around its pKa and at higher pH values. pDMAEMA inhibited bacterial growth by binding to the outside of the bacteria, permeabilising the outer membrane and disrupting the cytoplasmic membrane. By incorporating pDMAEMA with erythromycin, it was found that the efficacy of the latter was increased against Gram-negative bacteria. Together, the results illustrate that pDMAEMA acts in a similar fashion to other cationic biocides.
Funding Details: Science Foundation Ireland
Type of material: Journal Article
Publisher: American Chemical Society
Journal: Biomacromolecules
Volume: 11
Issue: 2
Start page: 443
End page: 453
Copyright (published version): 2009 American Chemical Society
Keywords: Poly(2-(dimethylamino ethyl)methacrylate))Antibacterial
Subject LCSH: Anti-infective agents
Polymers in medicine
Iatrogenic diseases--Prevention
Iatrogenic Disease--prevention & control
DOI: 10.1021/bm901166y
Other versions:
Language: en
Status of Item: Peer reviewed
ISSN: 1525-7797
This item is made available under a Creative Commons License:
Appears in Collections:Irish Drug Delivery Network Research Collection

Show full item record

Citations 1

Last Week
Last month
checked on Sep 11, 2020

Page view(s) 20

Last Week
Last month
checked on Oct 4, 2022

Download(s) 20

checked on Oct 4, 2022

Google ScholarTM



If you are a publisher or author and have copyright concerns for any item, please email and the item will be withdrawn immediately. The author or person responsible for depositing the article will be contacted within one business day.