Experimental and theoretical approach to comparative nanoparticle and small molecule intracellular import, trafficking, and export

Files in This Item:
File Description SizeFormat 
Salvati_Nanomed_revision_110119FINAL.pdfArticle447.76 kBAdobe PDFDownload
JN2010284R1.pdfFigures877.14 kBAdobe PDFDownload
Title: Experimental and theoretical approach to comparative nanoparticle and small molecule intracellular import, trafficking, and export
Authors: Salvati, Anna
Åberg, Christoffer
dos Santos, Tiago
Varela, Juan
Pinto, Paulo
Lynch, Iseult
Dawson, Kenneth A.
Permanent link: http://hdl.handle.net/10197/3340
Date: Dec-2011
Online since: 2011-11-24T15:07:23Z
Abstract: Central to understanding how nanoscale objects interact with living matter is the need for reproducible and verifiable data that can be interpreted with confidence. Likely this will be the basis of durable advances in nanomedicine and nanomedical safety. To develop these fields, there is also considerable interest in advancing the first generation of theoretical models of nanoparticle (NP) uptake into cells, and NP biodistribution in general. Here we present an uptake study comparing the outcomes for free molecular dye and NPs labeled with the same dye. A simple flux-based approach is presented to model NP uptake. We find that the intracellular NP concentration grows linearly in time, and that the uptake is essentially irreversible, with the particles accumulating in lysosomes. A wide range of practical challenges, from labile dye release to NP aggregation and the need to account for cell division, are addressed to ensure that these studies yield meaningful kinetic information.
Funding Details: Science Foundation Ireland
Irish Research Council for Science, Engineering and Technology
Type of material: Journal Article
Publisher: Elsevier
Journal: Nanomedicine: Nanotechnology, Biology and Medicine
Volume: 7
Issue: 6
Start page: 818
End page: 826
Copyright (published version): 2011 Elsevier Inc.
Keywords: Nanoparticle uptakeKineticsFlow cytometryFlux-based phenomenological model
Subject LCSH: Nanoparticles
Chemical kinetics
Flow cytometry
DOI: 10.1016/j.nano.2011.03.005
Other versions: http://dx.doi.org/10.1016/j.nano.2011.03.005
Language: en
Status of Item: Peer reviewed
Appears in Collections:Centre for Bionano Interactions (CBNI) Research Collection
Chemistry Research Collection

Show full item record

Citations 1

Last Week
Last month
checked on Feb 18, 2019

Google ScholarTM



This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. For other possible restrictions on use please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.