Role of cell cycle on the cellular uptake and dilution of nanoparticles in a cell population

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Title: Role of cell cycle on the cellular uptake and dilution of nanoparticles in a cell population
Authors: Kim, Jong Ah
Åberg, Christoffer
Salvati, Anna
Dawson, Kenneth A.
Permanent link: http://hdl.handle.net/10197/3695
Date: Jan-2012
Abstract: Nanoparticles are considered a primary vehicle for targeted therapies because they can pass biological barriers, enter and distribute in cells by energy-dependent pathways1-3. Until now, most studies have shown that nanoparticle properties, such as size4-6 and surface7,8, can affect how cells internalise nanoparticles. Here we show that the different phases of cell growth, which constitute the cell cycle, can also influence nanoparticle uptake. Although cells in different cell cycle phases internalised nanoparticles with similar rates, after 24 hours of uptake the concentration of nanoparticles in the cells is ranked according to the different cell cycle phases: G2/M > S > G0/G1. Nanoparticles were not exported from cells but the internalised nanoparticle concentration is split when the cell divides. Our results suggest that future studies on nanoparticle uptake should consider the cell cycle because in a cell population, the internalised nanoparticle dose in each cell varies as the cell cycles.
Funding Details: Science Foundation Ireland
Higher Education Authority
Irish Research Council for Science, Engineering and Technology
European Research Council
Type of material: Journal Article
Publisher: Nature Publishing Group
Copyright (published version): © 2011 Nature Publishing Group
Keywords: Nanoparticles;Cell cycle
Subject LCSH: Nanoparticles
Nanomedicine
Cell cycle
DOI: 10.1038/nnano.2011.191
Language: en
Status of Item: Peer reviewed
Appears in Collections:Centre for Bionano Interactions (CBNI) Research Collection
Conway Institute Research Collection
Chemistry Research Collection

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