Purification and properties of fluoroacetate dehalogenase from Pseudomonas fluorescens DSM 8341
|Title:||Purification and properties of fluoroacetate dehalogenase from Pseudomonas fluorescens DSM 8341||Authors:||Donnelly, Clár
Murphy, Cormac D.
|Permanent link:||http://hdl.handle.net/10197/3745||Date:||2009||Online since:||2012-08-17T14:18:59Z||Abstract:||The degradation of fluoroacetate by microorganisms has been established for some time, although only a handful of dehalogenases capable of hydrolyzing the stable C-F bond have been studied. The bacterium Pseudomonas fluorescens DSM 8341 was originally isolated from soil and very readily degraded fluoroacetate, thus it was thought that its dehalogenase might have some desirable properties. The enzyme was purified from cell free extracts and characterised: it is a monomer of 32,500 Da, with a pH optimum of 8 and is stable between pH 4 and 10; its activity is stimulated by some metal ions (Mg2+, Mn2+ and Fe3+), but inhibited by others (Hg2+, Ag2+). The enzyme is specific for fluoroacetate, and the Km for this substrate (0.68 mM) is the lowest determined for enzymes of this type that have been investigated to date.||Funding Details:||Other funder||Type of material:||Journal Article||Publisher:||Springer||Journal:||Biotechnology Letters||Volume:||31||Issue:||2||Start page:||245||End page:||250||Copyright (published version):||2008 Springer Science+Business Media B.V.||Keywords:||Dehalogenation; Fluoroacetate; Fluorocitrate; Pseudomonas fluorescens||Subject LCSH:||Halogen compounds
|DOI:||10.1007/s10529-008-9849-4||Language:||en||Status of Item:||Peer reviewed|
|Appears in Collections:||Biomolecular and Biomedical Science Research Collection|
CSCB Research Collection
Show full item record
This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. For other possible restrictions on use please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.