Effects of the Presence or Absence of a Protein Corona on Silica Nanoparticle Uptake and Impact on Cells

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Title: Effects of the Presence or Absence of a Protein Corona on Silica Nanoparticle Uptake and Impact on Cells
Authors: Lesniak, Anna
Fenaroli, Federico
Monopoli, Marco P.
Åberg, Christoffer
Dawson, Kenneth A.
Salvati, Anna
Permanent link: http://hdl.handle.net/10197/3830
Date: 21-Jun-2012
Abstract: Nanoparticles enter cells through active processes, thanks to their capability of interacting with the cellular machinery. The protein layer (corona) that forms on their surface once nanoparticles are in contact with biological fluids, such as the cell serum, mediates the interactions with cells in situ. As a consequence of this, here we show that the same nanomaterial can lead to very different biological outcomes, when exposed to cells in the presence or absence of a preformed corona. In particular, silica nanoparticles exposed to cells in the absence of serum have a stronger adhesion to the cell membrane and higher internalization efficiency, in comparison to what is observed in medium containing serum, when a preformed corona is present on their surface. The different exposure conditions not only affect the uptake levels but also result in differences in the intracellular nanoparticle location and impact on cells. Interestingly, we also show that after only one hour of exposure, a corona of very different nature forms on the nanoparticles exposed to cells in the absence of serum. Evidence suggests that these different outcomes can all be connected to the different adhesion and surface properties in the two conditions.
Funding Details: Irish Research Council for Science, Engineering and Technology
European Research Council
Type of material: Journal Article
Publisher: ACS Publications
Journal: ACS Nano
Volume: 6
Issue: 7
Start page: 5845
End page: 5857
Copyright (published version): 2012 American Chemical Society
Keywords: NanoparticleSilicaSerum freeProtein coronaNanoparticle uptakeAdhesion
Subject LCSH: Nanoparticles
Serum-free culture media
DOI: 10.1021/nn300223w
Language: en
Status of Item: Peer reviewed
Appears in Collections:Centre for Bionano Interactions (CBNI) Research Collection
Chemistry Research Collection

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