Amplification success of multilocus genotypes from feathers found in the field compared with feathers obtained from shot birds

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Title: Amplification success of multilocus genotypes from feathers found in the field compared with feathers obtained from shot birds
Authors: Johansson, Magnus P.
McMahon, Barry J.
Höglund, Jacob
Segelbacher, Gernot
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Date: Jan-2012
Online since: 2013-01-14T16:05:27Z
Abstract: Effective DNA extraction methods from bird feathers have facilitated non-invasive sampling, leading to the suggestion that feathers are a great source for genetic studies. However, few studies have assessed whether all feathers can be used or provide equal numbers of useful templates. In this study, feathers collected in various ways from Red Grouse Lagopus lagopus were examined to establish the quality of DNA extracted. Individual samples were classified into two categories according to whether they were collected from shot birds or found in the field. DNA was extracted from all samples and genotyped at 19 microsatellite loci. PCR products were analysed on a MegaBACE 1000. A total of 93% of the ‘shot’ category produced a genotype that was considered successful (i.e. 15 of 18 loci) and 23% of the ‘collected’ category produced successful genotypes under the same criteria. There was a significant difference between shot and collected samples in genotyping success and the observed number of missing loci. Recommendations and best practices are discussed along with the utility of bird feathers as a source of DNA for population and conservation biology.
Type of material: Journal Article
Publisher: Wiley-Blackwell
Journal: Ibis
Volume: 154
Issue: 1
Start page: 15
End page: 20
Copyright (published version): 2011 The Author(s) and 2011 British Ornithologists Union
Keywords: Genotyping successPCR amplificationRed Grouse Lagopus lagopus hibernicus
DOI: 10.1111/j.1474-919X.2011.01194.x
Language: en
Status of Item: Peer reviewed
Appears in Collections:Agriculture and Food Science Research Collection

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