Effect of organic solvents on the activity and stability of halophilic alcohol dehydrogenase (ADH2) from Haloferax volcanii

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Title: Effect of organic solvents on the activity and stability of halophilic alcohol dehydrogenase (ADH2) from Haloferax volcanii
Authors: Alsafadi, Diya
Paradisi, Francesca
Permanent link: http://hdl.handle.net/10197/4152
Date: 1-Jan-2013
Online since: 2014-01-01T04:00:07Z
Abstract: The effect of various organic solvents on the catalytic activity, stability and substrate specificity of alchohol dehydrogenase from Haloferax volcanii (HvADH2) was evaluated. The HvADH2 showed remarkable stability and catalysed the reaction in aqueous–organic medium containing dimethyl sulfoxide (DMSO) and methanol (MeOH). Tetrahydrofuran and acetonitrile were also investigated and adversely affected the stability of the enzyme. High concentration of salt, essential to maintain the enzymatic activity and structural integrity of the halophilic enzyme under standard conditions may be partially replaced by DMSO and MeOH. The presence of organic solvents did not induce gross changes in substrate specificity. DMSO offered a protective effect for the stability of the enzyme at nonoptimal pHs such as 6 and 10. Salt and solvent effects on the HvADH2 conformation and folding were examined through fluorescence spectroscopy. The fluorescence findings were consistent with the activity and stability results and corroborated the denaturing properties of some solvents. The intrinsic tolerance of this enzyme to organic solvent makes it highly attractive to industry.
Type of material: Journal Article
Publisher: Springer
Journal: Extremophiles
Volume: 17
Issue: 1
Start page: 115
End page: 122
Copyright (published version): 2013, Springer
Keywords: Halophilic enzymeAlcohol dehydrogenaseHaloferax volcaniiOrganic solvents toleranceFluorescence
DOI: 10.1007/s00792-012-0498-0
Language: en
Status of Item: Not peer reviewed
Appears in Collections:Chemistry Research Collection

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