Opioid mediated activity and expression of mu and delta opioid receptors in isolated human term non-laboring myometrium

Files in This Item:
File Description SizeFormat 
EJPharm_revision_JOC.pdf1.32 MBAdobe PDFDownload
Title: Opioid mediated activity and expression of mu and delta opioid receptors in isolated human term non-laboring myometrium
Authors: Fanning, Rebecca A.
McMorrow, Jason P.
Campion, Deirdre P.
Carey, Michael F.
O'Connor, J. J.
Permanent link: http://hdl.handle.net/10197/4207
Date: 5-Jan-2013
Abstract: The existence of opioid receptors in mammalian myometrial tissue is now widely accepted. Previously enkephalin degrading enzymes have been shown to be elevated in pregnant rat uterus and a met-enkephalin analogue has been shown to alter spontaneous contractility of rat myometrium. Here we have undertaken studies to determine the effects of met-enkephalin on in vitro human myometrial contractility and investigate the expression of opioid receptors in pregnant myometrium. Myometrial biopsies were taken from women undergoing elective caesarean delivery at term. Organ bath experiments were used to investigate the effect of the met-enkephalin analogue [d-Ala 2, d-met 5] enkephalin (DAMEA) on spontaneous contractility. A confocal immunofluorescent technique and real time PCR were used to determine the expression of protein and mRNA, respectively for two opioid receptor subtypes, mu and delta. DAMEA had a concentration dependent inhibitory effect on contractile activity (1×10−7 M–1×10−4 M; 54% reduction in contractile activity, P<0.001 at 1×10−4 M concentration). Mu and delta opioid receptor protein sub-types and their respective mRNA were identified in all tissues sampled. This is the first report of opioid receptor expression and of an opioid mediated uterorelaxant action in term human non-labouring myometrium in vitro
Type of material: Journal Article
Publisher: Elsevier
Journal: European Journal of Pharmacology
Volume: 698
Issue: 1-3
Start page: 170
End page: 177
Copyright (published version): 2012 Elsevier B.V.
Keywords: Human myometriumContractile activityPregnancyEndogenous opioid peptideOpioid receptor
DOI: 10.1016/j.ejphar.2012.09.045
Language: en
Status of Item: Peer reviewed
Appears in Collections:Conway Institute Research Collection
SBI Research Collection
Biomolecular and Biomedical Science Research Collection
Veterinary Medicine Research Collection

Show full item record

Citations 50

Last Week
Last month
checked on Oct 11, 2018

Google ScholarTM



This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. For other possible restrictions on use please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.