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A new microplate procedure for simultaneous assessment of lipophilic and hydrophilic antioxidants and pro-oxidants, using crocin and β-carotene bleaching methods in a single combined assay: Tea extracts as a case study
Date Issued
2013-10
Date Available
2013-04-08T15:24:43Z
Abstract
β-Carotene and crocin bleaching reactions are the basis of two methods extensively used to quantify antioxidant and pro-oxidant activities. They are appropriate for lipophilic and hydrophilic matrices, respectively, and can provide useful complementary information in the study of complex natural extracts containing components with variable degrees of polarity. In this regard, a microplate procedure (Carotene Combined Bleaching) is proposed that enables the combination of both methods in a single, informative and less expensive method which is also faster to carry out. As an illustrative model, the method was applied to test a set of commercial lipophilic and hydrophilic antioxidants and some predictable pro-oxidant agents. Afterwards, as a food compound case study, the antioxidant activity of five types of tea extracts (Green, Blue, White, Black and Red) were characterized and their equivalent potential activity was calculated using commercial antioxidants on the basis of the new procedure developed in this research. The activity of the tea extracts decreased in the following order: (a) In a predominantly lipophilic environment: White > Black > Red > Blue > Green tea extracts; and (b) In a predominantly hydrophilic environment: Green > Red > White > Black > Blue tea extracts
Sponsorship
Other funder
Other Sponsorship
CSIC (Intramural project: 200930I183) and Ministerio de Ciencia e Innovación (project CTM2010-18411, co-financed with FEDER funds by the European Union). JAE predoctoral program co-financed by the CSIC and European Social Fund (ESF).
Type of Material
Journal Article
Publisher
Elsevier
Journal
Food Research International
Volume
53
Issue
2
Start Page
836
End Page
846
Copyright (Published Version)
2012, Elsevier Ltd.
Language
English
Status of Item
Peer reviewed
This item is made available under a Creative Commons License
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New microplate procedure for antioxidants pro-oxidants lipophilic hydrophilic tea final.pdf
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1.29 MB
Format
Owning collection
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