A new microplate procedure for simultaneous assessment of lipophilic and hydrophilic antioxidants and pro-oxidants, using crocin and β-carotene bleaching methods in a single combined assay: Tea extracts as a case study

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Title: A new microplate procedure for simultaneous assessment of lipophilic and hydrophilic antioxidants and pro-oxidants, using crocin and β-carotene bleaching methods in a single combined assay: Tea extracts as a case study
Authors: Prieto Lage, Miguel Ángel
Murado García, Miguel Anxo
Vázquez Álvarez, José Antonio
Anders, Yvonne
Curran, Thomas P.
Permanent link: http://hdl.handle.net/10197/4236
Date: Oct-2013
Abstract: β-Carotene and crocin bleaching reactions are the basis of two methods extensively used to quantify antioxidant and pro-oxidant activities. They are appropriate for lipophilic and hydrophilic matrices, respectively, and can provide useful complementary information in the study of complex natural extracts containing components with variable degrees of polarity. In this regard, a microplate procedure (Carotene Combined Bleaching) is proposed that enables the combination of both methods in a single, informative and less expensive method which is also faster to carry out. As an illustrative model, the method was applied to test a set of commercial lipophilic and hydrophilic antioxidants and some predictable pro-oxidant agents. Afterwards, as a food compound case study, the antioxidant activity of five types of tea extracts (Green, Blue, White, Black and Red) were characterized and their equivalent potential activity was calculated using commercial antioxidants on the basis of the new procedure developed in this research. The activity of the tea extracts decreased in the following order: (a) In a predominantly lipophilic environment: White > Black > Red > Blue > Green tea extracts; and (b) In a predominantly hydrophilic environment: Green > Red > White > Black > Blue tea extracts
Funding Details: Other funder
Type of material: Journal Article
Publisher: Elsevier
Copyright (published version): 2012, Elsevier Ltd.
Keywords: Antioxidant activityβ-Carotene methodCrocin bleaching methodMicroplate analysisAntioxidant activity of tea extractsNon-linear responsesMathematical modeling
DOI: 10.1016/j.foodres.2012.11.026
Language: en
Status of Item: Peer reviewed
Appears in Collections:Biosystems and Food Engineering Research Collection

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