Protein disorder and short conserved motifs in disordered regions are enriched near the cytoplasmic side of single-pass transmembrane proteins

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Title: Protein disorder and short conserved motifs in disordered regions are enriched near the cytoplasmic side of single-pass transmembrane proteins
Authors: Stavropoulos, Ilias
Khaldi, Nora
Davey, Norman E.
O'Brien, Kevin
Martin, Finian
Shields, Denis C.
Permanent link: http://hdl.handle.net/10197/4242
Date: 4-Sep-2012
Abstract: Intracellular juxtamembrane regions of transmembrane proteins play pivotal roles in cell signalling, mediated by protein-protein interactions. Disordered protein regions, and short conserved motifs within them, are emerging as key determinants of many such interactions. Here, we investigated whether disorder and conserved motifs are enriched in the juxtamembrane area of human single-pass transmembrane proteins. Conserved motifs were defined as short disordered regions that were much more conserved than the adjacent disordered residues. Human single-pass proteins had higher mean disorder in their cytoplasmic segments than their extracellular parts. Some, but not all, of this effect reflected the shorter length of the cytoplasmic tail. A peak of cytoplasmic disorder was seen at around 30 residues from the membrane. We noted a significant increase in the incidence of conserved motifs within the disordered regions at the same location, even after correcting for the extent of disorder. We conclude that elevated disorder within the cytoplasmic tail of many transmembrane proteins is likely to be associated with enrichment for signalling interactions mediated by conserved short motifs.
Type of material: Journal Article
Publisher: PLOS
Copyright (published version): 2012, Stavropoulos et al.
Keywords: Integral membrane proteins;Intracellular membranes;Membrane potential;Membrane proteins;Membrane receptor signalling
DOI: 10.1371/journal.pone.0044389
Language: en
Status of Item: Peer reviewed
Appears in Collections:Medicine Research Collection

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