Influence of surface functionality of poly(propylene imine) dendrimers on protease resistance and propagation of the scrapie prion protein

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Title: Influence of surface functionality of poly(propylene imine) dendrimers on protease resistance and propagation of the scrapie prion protein
Authors: Fischer, Marlies
Appelhans, Dietmar
Schwarz, Simona
Klajnert, Barbara
Bryszewska, Maria
Voit, Brigitte
Rogers, Mark S.
Permanent link: http://hdl.handle.net/10197/4250
Date: 20-Apr-2010
Abstract: Accumulation of PrP(Sc), an insoluble and protease-resistant pathogenic isoform of the cellular prion protein (PrP(C)), is a hallmark in prion diseases. Branched polyamines, including PPI (poly(propylene imine)) dendrimers, are able to remove protease resistant PrP(Sc) and abolish infectivity, offering possible applications for therapy. These dendrimer types are thought to act through their positively charged amino surface groups. In the present study, the molecular basis of the antiprion activity of dendrimers was further investigated, employing modified PPI dendrimers in which the positively charged amino surface groups were substituted with neutral carbohydrate units of maltose (mPPI) or maltotriose (m3PPI). Modification of surface groups greatly reduced the toxicity associated with unmodified PPI but did not abolish its antiprion activity, suggesting that the presence of cationic surface groups is not essential for dendrimer action. PPI and mPPI dendrimers of generation 5 were equally effective in reducing levels of protease-resistant PrP(Sc) (PrP(res)) in a dose- and time-dependent manner in ScN2a cells and in pre-existing aggregates in homogenates from infected brain. Solubility assays revealed that total levels of PrP(Sc) in scrapie-infected mouse neuroblastoma (ScN2a) cells were reduced by mPPI. Coupled with the known ability of polyamino dendrimers to render protease-resistant PrP(Sc) in pre-existing aggregates of PrP(Sc) susceptible to proteolysis, these findings strongly suggest that within infected cells dendrimers reduce total amounts of PrP(Sc) by mediating its denaturation and subsequent elimination.
Type of material: Journal Article
Publisher: American Chemical Society
Journal: Biomacromolecules
Volume: 11
Issue: 5
Start page: 1314
End page: 1325
Copyright (published version): 2010 American Chemical Society
Keywords: DendrimersPrionsPrPScPrion proteinAggregationGlycodendrimerPolyamino dendrimer
DOI: 10.1021/bm100101s
Other versions: http://pubs.acs.org/doi/abs/10.1021/bm100101s
Language: en
Status of Item: Peer reviewed
Appears in Collections:Biology & Environmental Science Research Collection

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