Detachment characteristics of a mixed culture biofilm using particle size analysis
Title: | Detachment characteristics of a mixed culture biofilm using particle size analysis | Authors: | Walter, Maik Safari, Ashkan Ivankovic, Alojz Casey, Eoin |
Permanent link: | http://hdl.handle.net/10197/4370 | Date: | 15-Jul-2013 | Online since: | 2013-06-18T11:54:06Z | Abstract: | Detachment is a critically important aspect of biofilm processes; it impacts not only on the characteristics of the biofilm itself but also has general implications for the dissemination of pathogenic bacteria and the operation of biofilm reactors. The mechanisms of biofilm detachment are of fundamental importance in the analysis of biofilm processes. However the complexity of biofilm detachment creates difficulties in performing and analyzing experiments. It is necessary to identify if, under steady conditions, biofilms experiments are reproducible with respect to detachment. In this study mixed culture biofilms were cultivated under low shear conditions over four days in glass flow cells in triplicate under non-recirculation conditions. Detached particles were regularly sampled, were stained, filtered and analyzed using a fluorescence microscope to establish size distributions of detached cells and cell clumps. This study has shown that, despite the existence of a complex particle size distribution, reproducibility is possible in four day old mixed culture biofilms. This has important implications for the study of active or passive detachment in biofilm systems. This study also distinguished between erosion and sloughing following step increases in shear stress. | Funding Details: | Science Foundation Ireland | Type of material: | Journal Article | Publisher: | Elsevier | Journal: | Chemical Engineering Journal | Volume: | 228 | Issue: | 15 July 2013 | Start page: | 1140 | End page: | 1147 | Copyright (published version): | 2013 Published by Elsevier B.V. | Keywords: | Biofilm; Detachment; Erosion; Sloughing; Shear; Size-analysis | DOI: | 10.1016/j.cej.2013.05.071 | Other versions: | http://dx.doi.org/10.1016/j.cej.2013.05.071 | Language: | en | Status of Item: | Peer reviewed |
Appears in Collections: | Chemical and Bioprocess Engineering Research Collection |
Show full item record
SCOPUSTM
Citations
20
16
Last Week
0
0
Last month
checked on Feb 20, 2019
Page view(s) 10
211
checked on May 25, 2018
Download(s) 20
301
checked on May 25, 2018
Google ScholarTM
Check
Altmetric
This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. For other possible restrictions on use please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.