Beyond the diffraction limit : far-field fluorescence imaging with ultrahigh resolution

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Title: Beyond the diffraction limit : far-field fluorescence imaging with ultrahigh resolution
Authors: Rice, James H.
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Date: Nov-2007
Online since: 2013-07-22T14:22:51Z
Abstract: Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution has stimulated research into developing methods to image beyond the diffraction limit based on far-field fluorescence microscopy techniques. Rapid progress is being made in this area of science with methods emerging that enable fluorescence imaging in the far-field to possess a resolution well beyond the diffraction limit. This review outlines developments in far-field fluorescence methods which enable ultrahigh resolution imaging and application of these techniques to biology. Future possible trends and directions in far-field fluorescence imaging with ultrahigh resolution are also outlined.
Type of material: Journal Article
Publisher: RSC Publishing
Journal: Molecular BioSystems
Volume: 3
Issue: 11
Start page: 781
End page: 793
Copyright (published version): 2007, The Royal Society of Chemistry
Keywords: Nano-IRInfrared absorptionNanoimagingNanoscopyImagingChemical mappingAFM
DOI: 10.1039/B705460B
Language: en
Status of Item: Peer reviewed
Appears in Collections:Physics Research Collection

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