All or Nothing : protein complexes flip essentiality between distantly related eukaryotes

Files in This Item:
File Description SizeFormat 
Genome_Biol_Evol-2013-Ryan-1049-59.pdf461.19 kBAdobe PDFDownload
Title: All or Nothing : protein complexes flip essentiality between distantly related eukaryotes
Authors: Ryan, Colm J.
Krogan, Nevan J.
Cunningham, Pádraig
Cagney, Gerard
Permanent link: http://hdl.handle.net/10197/4492
Date: 8-May-2013
Abstract: In the budding yeast Saccharomyces cerevisiae, the subunits of any given protein complex are either mostly essential or mostly nonessential, suggesting that essentiality is a property of molecular machines rather than individual components. There are exceptions to this rule, however, that is, nonessential genes in largely essential complexes and essential genes in largely nonessential complexes. Here, we provide explanations for these exceptions, showing that redundancy within complexes, as revealed by genetic interactions, can explain many of the former cases, whereas "moonlighting," as revealed by membership of multiple complexes, can explain the latter. Surprisingly, we find that redundancy within complexes cannot usually be explained by gene duplication, suggesting alternate buffering mechanisms. In the distantly related Schizosaccharomyces pombe, we observe the same phenomenon of modular essentiality, suggesting that it may be a general feature of eukaryotes. Furthermore, we show that complexes flip essentiality in a cohesive fashion between the two species, that is, they tend to change from mostly essential to mostly nonessential, or vice versa, but not to mixed patterns. We show that these flips in essentiality can be explained by differing lifestyles of the two yeasts. Collectively, our results support a previously proposed model where proteins are essential because of their involvement in essential functional modules rather than because of specific topological features such as degree or centrality.
Type of material: Journal Article
Publisher: Oxford University Press
Copyright (published version): The Author(s) 2013
Keywords: Essentiality;Modularity;Yeast;Redundancy;Genetic interactions;Protein complexes
DOI: 10.1093/gbe/evt074
Language: en
Status of Item: Peer reviewed
Appears in Collections:Computer Science Research Collection
Biomolecular and Biomedical Science Research Collection

Show full item record

SCOPUSTM   
Citations 20

10
Last Week
0
Last month
checked on Jun 22, 2018

Google ScholarTM

Check

Altmetric


This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. For other possible restrictions on use please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.