Options
Nanoscale infrared absorption imaging permits non-destructive intracellular photosensitizer localization for subcellular uptake analysis
Alternative Title
Nanoscale infrared spectral imaging for non-invasive intracellular gold nanoparticle conjugate localization
Date Issued
2013-06-07
Date Available
2014-06-07T03:00:08Z
Abstract
The most immediate biological and medical advantages of therapeutic agent localization on the nanoscale arise from the increased understanding of targeted delivery, selectivity and intracellular distribution that are gained by imaging at the resolution scale of individual nanovectors and therapeutic agents themselves. This paper reports on the use of a nanoscale resolution chemical imaging method, infrared (IR) nanospectral absorption imaging, used to map the subcellular localization of a photoactive therapeutic agent - toluidine blue-conjugated gold nanoparticles (TBO) within nanoscale subsections of single colon adenocarcinoma cells. By comparison of photosensitizer distribution with diffraction limited optical imaging, the benefits of IR nanospectral localization are highlighted and the spatial and spectral accuracy of the non-destructive IR imaging method is confirmed. IR spectral ratio imaging is presented as a means to map intracellular nanoparticle density at sub 50 nm lateral resolution with IR nanospectroscopy enabling distinction of nanoparticle seeded cells from a control group with 95% confidence. In this way we illustrate that IR absorption nanoimaging combined with IR point source data does not only yield intracellular drug detection on the order of nanometres, but also permits extension of the AFM-IR technique from subcellular analysis up to studies of cell numbers that are statistically significant.
Type of Material
Journal Article
Publisher
RSC Publishing
Journal
RSC Advances
Volume
3
Issue
33
Start Page
13789
End Page
13795
Copyright (Published Version)
Royal Society of Chemistry 2013
Language
English
Status of Item
Peer reviewed
This item is made available under a Creative Commons License
File(s)
Owning collection
Scopus© citations
29
Acquisition Date
Mar 19, 2024
Mar 19, 2024
Views
1824
Last Month
1
1
Acquisition Date
Mar 19, 2024
Mar 19, 2024
Downloads
518
Last Week
6
6
Last Month
13
13
Acquisition Date
Mar 19, 2024
Mar 19, 2024