Disinfection of a polyamide nanofiltration membrane using ethanol

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Title: Disinfection of a polyamide nanofiltration membrane using ethanol
Authors: Heffernan, Rory
Correia-Semião, Andrea Joana C.
Desmond, P.
Cao, Huayu
Safari, Ashkan
Habimana, Olivier
Casey, Eoin
Permanent link: http://hdl.handle.net/10197/4556
Date: 15-Dec-2013
Abstract: It is imperative that nanofiltration membranes are disinfected before they are used for laboratory-scale bacterial adhesion or biofouling experiments, yet currently no suitable disinfection protocol exists. This study aimed to determine if an ethanol treatment at a minimum inhibitory concentration (MIC) could be used to effectively disinfect nanofiltration membranes without altering membrane properties which could affect research. Two strains of bacteria, Pseudomonas fluorescens and Staphylococcus sp., were exposed to a range of ethanol concentrations to determine the MIC required for a 4log10 reduction in bacteria. In parallel, ethanol's effects on the filtration, surface and mechanical properties of a Dow Filmtec NF90 membrane were analysed. A 1.5 hour treatment with 40% ethanol was shown to effectively disinfect the membrane without significantly affecting any of the membranes properties tested. This treatment protocol can now be safely used to disinfect the studied membrane prior to bacterial adhesion or biofouling experiments. This study also acts as a guideline for researchers using other membranes to determine a suitable disinfection protocol for their needs.
Funding Details: European Research Council
Science Foundation Ireland
Type of material: Journal Article
Publisher: Elsevier
Journal: Journal of Membrane Science
Volume: 448
Issue: 15 December 2013
Start page: 170
End page: 179
Copyright (published version): 2013 Published by Elsevier B.V.
Keywords: DisinfectionEthanolPre-treatmentMICNanofiltration
DOI: 10.1016/j.memsci.2013.07.069
Language: en
Status of Item: Peer reviewed
Appears in Collections:Chemical and Bioprocess Engineering Research Collection

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