Progesterone-induced blocking factor differentially regulates trophoblast and tumor invasion by altering matrix metalloproteinase activity

DC FieldValueLanguage
dc.contributor.authorHalasz, Melindaen
dc.contributor.authorPolgar, Beataen
dc.contributor.authorBerta, Gergelyen
dc.contributor.authoret al.en
dc.date.accessioned2013-11-28T15:07:58Z-
dc.date.available2014-06-27T03:00:08Z-
dc.date.copyright2013 Springer-Verlagen
dc.date.issued2013-06-27en
dc.identifier.citationCellular and Molecular Life Sciencesen
dc.identifier.urihttp://hdl.handle.net/10197/5005-
dc.description.abstractInvasiveness is a common feature of trophoblast and tumors; however, while tumor invasion is uncontrolled, trophoblast invasion is strictly regulated. Both trophoblast and tumor cells express high levels of the immunomodulatory progesterone-induced blocking factor (PIBF), therefore, we aimed to test the possibility that PIBF might be involved in invasion. To this aim, we used PIBF-silenced or PIBF-treated trophoblast (HTR8/Svneo, and primary trophoblast) and tumor (HT-1080, A549, HCT116, PC3) cell lines. Silencing of PIBF increased invasiveness as well as MMP-2,-9 secretion of HTR8/SVneo, and decreased those of HT-1080 cells. PIBF induced immediate STAT6 activation in both cell lines. Silencing of IL-4Rα abrogated all the above effects of PIBF, suggesting that invasion-related signaling by PIBF is initiated through the IL-4Rα/PIBF-receptor complex. In HTR-8/SVneo, PIBF induced fast, but transient Akt and ERK phosphorylation, whereas in tumor cells, PIBF triggered sustained Akt, ERK, and late STAT3 activation. The late signaling events might be due to indirect action of PIBF. PIBF induced the expression of EGF and HB-EGF in HT-1080 cells. The STAT3-activating effect of PIBF was reduced in HB-EGF-deficient HT-1080 cells, suggesting that PIBF-induced HB-EGF contributes to late STAT3 activation. PIBF binds to the promoters of IL-6, EGF, and HB-EGF; however, the protein profile of the protein/DNA complex is different in the two cell lines. We conclude that in tumor cells, PIBF induces proteins, which activate invasion signaling, while—based on our previous data—PIBF might control trophoblast invasion by suppressing proinvasive genes.en
dc.language.isoenen
dc.publisherSpringer-Verlagen
dc.rightsThe final publication is available at www.springerlink.comen
dc.subjectPIBFen
dc.subjectInvasionen
dc.subjectTumoren
dc.subjectTrophoblasten
dc.subjectZebrafishen
dc.titleProgesterone-induced blocking factor differentially regulates trophoblast and tumor invasion by altering matrix metalloproteinase activityen
dc.typeJournal Articleen
dc.internal.availabilityFull text availableen
dc.statusPeer revieweden
dc.identifier.volume70en
dc.identifier.issue23en
dc.identifier.startpage4617en
dc.identifier.endpage4630en
dc.identifier.doi10.1007/s00018-013-1404-3-
dc.neeo.contributorHalasz|Melinda|aut|-
dc.neeo.contributorPolgar|Beata|aut|-
dc.neeo.contributorBerta|Gergely|aut|-
dc.neeo.contributoret al.||aut|-
dc.internal.notesPaper118.pdfen
dc.description.othersponsorshipHungarian National Research Fund (OTKA 77717), from theUniversity of Pecs (34039/KA-PostDoc12-03) and by TÁMOP-4.2.1/B-10/1-2010-0002.en
dc.description.adminDeposited by bulk importen
dc.date.updated2013-11-27T16:35:50.274Zen
item.grantfulltextopen-
item.fulltextWith Fulltext-
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