Thermoresponsive Substrates used for the Expansion of Human Mesenchymal Stem Cells and the Preservation of Immunophenotype

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Title: Thermoresponsive Substrates used for the Expansion of Human Mesenchymal Stem Cells and the Preservation of Immunophenotype
Authors: Nash, Maria E.
Fan, Xingliang
Carroll, William M.
et al.
Permanent link: http://hdl.handle.net/10197/5018
Date: 26-Jan-2013
Abstract: The facile regeneration of undifferentiated human mesenchymal stem cells (hMSCs) from thermoresponsive surfaces facilitates the collection of stem cells avoiding the use of animal derived cell detachment agents commonly used in cell culture. This communication proposes a procedure to fabricate coatings from commercially available pNIPAm which is both affordable and a significant simplification on alternative approaches used elsewhere. Solvent casting was used to produce films in the micrometer range and successful cell adhesion and proliferation was highly dependent on the thickness of the coating produced with 1 μm thick coatings supporting cells to confluence. 3T3 cell sheets and hMSCs were successfully detached from the cast coatings upon temperature reduction. Furthermore, results indicate that the hMSCs remained undifferentiated as the surface receptor profile of hMSCs was not altered when cells were detached in this manner.
Type of material: Journal Article
Publisher: Springer-Verlag
Copyright (published version): 2013 Springer-Verlag
Keywords: Thermoresponsive polymers;Human mesenchymal stem cells;Cell expansion and regeneration;Flow cytometry;Stem cell differentiation
DOI: 10.1007/s12015-013-9428-5
Language: en
Status of Item: Peer reviewed
Appears in Collections:SBI Research Collection

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