Cell cycle-dependent formation of Cdc45-Claspin complexes in human cells is compromized by UV-mediated DNA damage

Files in This Item:
File Description SizeFormat 
Paper105.pdf2.45 MBAdobe PDFDownload
Title: Cell cycle-dependent formation of Cdc45-Claspin complexes in human cells is compromized by UV-mediated DNA damage
Authors: Broderick, Ronan
Rainey, Michael D.
Santocanale, Corrado
et al.
Permanent link: http://hdl.handle.net/10197/5053
Date: 27-Aug-2013
Online since: 2013-11-29T09:50:30Z
Abstract: The replication factor Cdc45 has essential functions in the initiation and elongation steps of eukaryotic DNA replication and plays an important role in the intra-S-phase checkpoint. Its interactions with other replication proteins during the cell cycle and after intra-S-phase checkpoint activation are only partially characterized. In the present study, we show that the C terminal part of Cdc45 may mediate its interactions with Claspin. The interactions of human Cdc45 with the three replication factors Claspin, replication protein A and DNA polymerase δ are maximal during the S phase. Following UVC-induced DNA damage, Cdc45–Claspin complex formation is reduced, whereas the binding of Cdc45 to replication protein A is not affected. We also show that treatment of cells with UCN-01 and phosphatidylinositol 3-kinase-like kinase inhibitors does not rescue the UV-induced destabilization of Cdc45–Claspin interactions, suggesting that the loss of the interaction between Cdc45 and Claspin occurs upstream of ataxia telangiectasia and Rad 3-related activation in the intra-S-phase checkpoint.
Type of material: Journal Article
Publisher: Wiley Blackwell (Blackwell Publishing)
Journal: FEBS Journal
Volume: 280
Issue: 19
Start page: 4888
End page: 4902
Copyright (published version): 2013 Wiley Blackwell (Blackwell Publishing)
Keywords: Cdc45claspinDNA damage responseDNA replicationintra-S-phase checkpoint
DOI: 10.1111/febs.12465
Language: en
Status of Item: Peer reviewed
Appears in Collections:SBI Research Collection

Show full item record

Citations 50

Last Week
Last month
checked on Feb 11, 2019

Page view(s) 50

checked on May 25, 2018

Download(s) 50

checked on May 25, 2018

Google ScholarTM



This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. For other possible restrictions on use please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.