Global proteomic characterization of uterine histotroph recovered from beef heifers yielding good quality and degenerate day 7 embryos

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Title: Global proteomic characterization of uterine histotroph recovered from beef heifers yielding good quality and degenerate day 7 embryos
Authors: Beltman, Marijke Eileen
Mullen, M. P.
Elia, G.
Hilliard, M.
Diskin, M. G.
Evans, A. C.
Crowe, Mark
Permanent link: http://hdl.handle.net/10197/5119
Date: Jan-2014
Abstract: The objective was to analyze the proteomic composition of uterine flushes collected from beef heifers on day 7 after insemination. Estrus was synchronized in crossbred beef heifers by using a protocol with a controlled intravaginal drug releasing device. Heifers detected in standing estrus (within 24-48 h after removal of controlled intravaginal drug releasing device) were inseminated (estrus = day 0) with frozen-thawed semen from a single ejaculate of a bull with proven fertility. Heifers from which an embryo was recovered (after slaughter on day 7) were classified as either having a viable embryo (morula/blastocyst stage) or a degenerate embryo (arrested at the 2- to 16-cell stage). The overall recovery rate (viable and degenerate combined) was 64%. Global liquid chromatography coupled to tandem mass spectrometry proteomic analysis of the histotroph collected identified 40 high-confidence proteins present on day 7; 26 proteins in the viable group, 10 in the degenerate group, and 4 shared between both groups. Five proteins (platelet-activating factor acetylhydrolase IB subunit γ [PAFAH1B3], tubulin α-1D chain, tubulin β-4A chain, cytochrome C, and dihydropyrimidinase-related protein-2) were unique or more abundant in the histotroph collected from animals with a viable embryo, and 1 protein (S100-A4) was more abundant in the histotroph collected from animals with a degenerate embryo. Of interest, PAFAH1B3, detected only in histotroph from the group yielding viable embryos, belongs to the group of platelet-activating factors that are known to be important for the development of the pre-implantation embryo in other species. To our knowledge this is the first report of PAFAH1B3 in relation to bovine early embryonic development.
Funding Details: Science Foundation Ireland
Type of material: Journal Article
Publisher: Elsevier
Copyright (published version): 2013 Elsevier
Keywords: Histotroph;Proteomics;Embryo development
DOI: 10.1016/j.domaniend.2013.10.003
Language: en
Status of Item: Peer reviewed
Appears in Collections:Veterinary Medicine Research Collection

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