The APSES transcription factor Efg1 is a global regulator that controls morphogenesis and biofilm formation in Candida parapsilosis
|Title:||The APSES transcription factor Efg1 is a global regulator that controls morphogenesis and biofilm formation in Candida parapsilosis||Authors:||Connolly, Leona A.
Holland, Linda M.
Lynch, Denise B.
|Permanent link:||http://hdl.handle.net/10197/5134||Date:||Oct-2013||Abstract:||Efg1 (a member of the APSES family) is an important regulator of hyphal growth and of the white-to-opaque transition in C. albicans and very closely related species. We show that in Candida parapsilosis Efg1 is a major regulator of a different morphological switch at the colony level, from a concentric to smooth morphology. The rate of switching is at least 20-fold increased in an efg1 knockout relative to wild type. Efg1 deletion strains also have reduced biofilm formation, attenuated virulence in an insect model, and increased sensitivity to SDS and caspofungin. Biofilm reduction is more dramatic in in vitro than in in vivo models. An Efg1 paralog (Efh1) is restricted to Candida species, and does not regulate concentric-smooth phenotype switching, biofilm formation or stress response. We used ChIP-seq to identify the Efg1 regulon. 931 promoter regions bound by Efg1 are highly enriched for transcription factors and regulatory proteins. Efg1 also binds to its own promoter, and negatively regulates its expression. Efg1 targets are enriched in binding sites for 93 additional transcription factors, including Ndt80. Our analysis suggests that Efg1 has an ancient role as regulator of development in fungi, and is central to several regulatory networks.||Funding Details:||Irish Research Council
Science Foundation Ireland
|Type of material:||Journal Article||Publisher:||Wiley||Copyright (published version):||2013 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.||Keywords:||Concentric morphology; Smooth morphology; Biofilm; Promoter regions; Biofilm development; Morphogenisis||DOI:||10.1111/mmi.12345||Language:||en||Status of Item:||Peer reviewed|
|Appears in Collections:||Biomolecular and Biomedical Science Research Collection|
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