Nanoparticle accumulation and transcytosis in brain endothelial cell layers

DC FieldValueLanguage
dc.contributor.authorYe, Dong
dc.contributor.authorNic Raghnaill, Michelle
dc.contributor.authorBramini, Mattia
dc.contributor.authorMahon, Eugene
dc.contributor.authorÅberg, Christoffer
dc.contributor.authorSalvati, Anna
dc.contributor.authorDawson, Kenneth A.
dc.date.accessioned2013-12-09T12:09:37Z
dc.date.available2014-09-01T03:00:07Z
dc.date.copyright2013 The Royal Society of Chemistryen
dc.date.issued2013-09
dc.identifier.citationNanoscaleen
dc.identifier.issn2040-3364
dc.identifier.urihttp://hdl.handle.net/10197/5140
dc.description.abstractThe blood–brain barrier (BBB) is a selective barrier, which controls and limits access to the central nervous system (CNS). The selectivity of the BBB relies on specialized characteristics of the endothelial cells that line the microvasculature, including the expression of intercellular tight junctions, which limit paracellular permeability. Several reports suggest that nanoparticles have a unique capacity to cross the BBB. However, direct evidence of nanoparticle transcytosis is difficult to obtain, and we found that typical transport studies present several limitations when applied to nanoparticles. In order to investigate the capacity of nanoparticles to access and transport across the BBB, several different nanomaterials, including silica, titania and albumin- or transferrin-conjugated gold nanoparticles of different sizes, were exposed to a human in vitro BBB model of endothelial hCMEC/D3 cells. Extensive transmission electron microscopy imaging was applied in order to describe nanoparticle endocytosis and typical intracellular localisation, as well as to look for evidence of eventual transcytosis. Our results show that all of the nanoparticles were internalised, to different extents, by the BBB model and accumulated along the endo–lysosomal pathway. Rare events suggestive of nanoparticle transcytosis were also observed for several of the tested materials.en
dc.description.sponsorshipScience Foundation Irelanden
dc.language.isoenen
dc.publisherRoyal Society of Chemistryen
dc.subjectBlood-brain barrier
dc.subjectTransport studies
dc.subjectNanomaterials
dc.titleNanoparticle accumulation and transcytosis in brain endothelial cell layersen
dc.typeJournal Articleen
dc.internal.authorcontactotherchristoffer.aberg@cbni.ucd.ie
dc.internal.availabilityFull text availableen
dc.statusPeer revieweden
dc.identifier.volume5en
dc.identifier.issue22en
dc.identifier.startpage11153en
dc.identifier.endpage11165en
dc.identifier.doi10.1039/C3NR02905K-
dc.neeo.contributorYe|Dong|aut|-
dc.neeo.contributorNic Raghnaill|Michelle|aut|-
dc.neeo.contributorBramini|Mattia|aut|-
dc.neeo.contributorMahon|Eugene|aut|-
dc.neeo.contributorÅberg|Christoffer|aut|-
dc.neeo.contributorSalvati|Anna|aut|-
dc.neeo.contributorDawson|Kenneth A.|aut|-
dc.description.othersponsorshipINSPIRE (Integrated NanoScience Platform for Ireland)en
dc.description.othersponsorshipEU FP7 Small Collaborative projects NeuroNanoen
dc.description.othersponsorshipNanoTransKineticsen
dc.description.adminDM, 9/12/2013en
dc.internal.rmsid369303286
dc.date.updated2013-11-19T17:01:13Z
dc.rights.licensehttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/en
item.grantfulltextopen-
item.fulltextWith Fulltext-
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