Production of lipopeptides in Bacillus sp. CS93 isolated from Pozol

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Title: Production of lipopeptides in Bacillus sp. CS93 isolated from Pozol
Authors: Moran, Stephen
Robertson, Keith
Paradisi, Francesca
Rai, Dilip K.
Murphy, Cormac D.
Permanent link: http://hdl.handle.net/10197/5179
Date: Mar-2010
Abstract: Bacillus sp. strain CS93, which was previously isolated from Pozol, was previously shown to produce iturin A, bacilysin and chlorotetaine. To investigate the biosynthetic mechanism of chlorotetaine production, the bac genes were amplified from genomic DNA of Bacillus sp. CS93 by PCR and sequenced. The genes bacABCDE were determined, but no gene that might code for a halogenating enzyme was detected either within the gene cluster or in the flanking sequences. Following further analysis of culture supernatants that were active against bacteria by liquid chromatography-MS, it was not possible to detect bacilysin/chlorotetaine. However, in methanolic fractions containing antibacterial activity, molecular ions characteristic of surfactins and fengycin were detectable by electrospray MS. Using primers complementary for conserved regions of nonribosomal peptide synthase, it was possible to amplify gene fragments that had a high degree of homology with known surfactin and fengycin biosynthetic genes. Thus, in addition to the known antimicrobial compounds, we have shown that this strain produces other bioactive lipopeptides, which might account for some of the medicinal properties of Pozol.
Type of material: Journal Article
Publisher: Wiley
Journal: FEMS Microbiology Letters
Volume: 304
Issue: 1
Start page: 69
End page: 73
Copyright (published version): 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd.
Keywords: Bacillus subtilisSurfactinMS
DOI: 10.1111/j.1574-6968.2009.01882.x
Language: en
Status of Item: Peer reviewed
Appears in Collections:Biomolecular and Biomedical Science Research Collection

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