Changes in the endometrial transcriptome during the bovine estrous cycle: effect of low circulating progesterone and consequences for conceptus elongation

Files in This Item:
File Description SizeFormat 
Forde_Beltman_et_al.pdf705.74 kBAdobe PDFDownload
Title: Changes in the endometrial transcriptome during the bovine estrous cycle: effect of low circulating progesterone and consequences for conceptus elongation
Authors: Forde, Niamh
Beltman, Marijke Eileen
Duffy, G. B.
Duffy, P.
Mehta, J. P.
Ó'Gaora, P.
Roche, J. F.
Lonergan, Patrick
Crowe, Mark
Permanent link: http://hdl.handle.net/10197/5265
Date: 1-Feb-2011
Abstract: In cattle, elevated concentrations of circulating progesterone (P4) in the immediate post-conception period have been associated with an advancement of conceptus elongation, an associated increase in interferon-tau production and higher pregnancy rates. Low P4 has been implicated as a causative factor in the low pregnancy rates observed in dairy cows. The aims of this study were (1) to describe the changes that occur in the bovine endometrial transcriptome during the estrous cycle, (2) to determine how elevated P4 affects the temporal pattern of gene expression in the endometrium of cyclic heifers, (3) to determine if the expression of these genes is altered in heifers with low P4 and (4) to determine the consequences of low P4 for conceptus development following embryo transfer. The main findings were that 1) relatively few differences occurred in endometrial gene expression during the early luteal phase of the estrous cycle under normal concentrations of P4 (Day 5 versus Day 7) but comparison of endometria from more distant stages of the luteal phase (Day 7 versus Day 13) revealed large transcriptional changes; 2) exogenous supplementation of P4, leading to elevated concentrations from Day 3 to Day 8, considerably altered the expression of a large number of genes at all stages of the luteal phase; 3) induction of low circulating P4 altered the normal temporal changes that occured in the expression of these genes, mainly by delaying their expression; 4) this delay in gene expression was, in part, due to delayed down regulation of the PGR from the LE and GE, and 5) the altered endometrial gene expression induced by low P4 was associated with a reduced capacity of the uterus to support conceptus development after embryo transfer on Day 7. In conclusion, the present study provides clear evidence for a temporal change in the transcriptomic signature of the bovine endometrium which is sensitive to the concentrations of circulating P4 in the first few days after estrus and which can, under conditions of low progesterone, lead to an suboptimal uterine environment and a reduced ability to support conceptus elongation.
Funding Details: Science Foundation Ireland
Type of material: Journal Article
Publisher: Society for the Study of Reproduction
Copyright (published version): 2011 The Society for the Study of Reproduction, Inc.
Keywords: Embryo;Endometrium;Gene expression;progesterone;Progesterone receptor
DOI: 10.1095/biolreprod.110.085910
Language: en
Status of Item: Peer reviewed
Appears in Collections:Veterinary Medicine Research Collection

Show full item record

SCOPUSTM   
Citations 1

137
Last Week
1
Last month
checked on Jun 23, 2018

Google ScholarTM

Check

Altmetric


This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. For other possible restrictions on use please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.