Influence of coating properties on the adhesion of proteins to atmospheric plasma modified surfaces

Title: Influence of coating properties on the adhesion of proteins to atmospheric plasma modified surfaces
Authors: Stallard, Charlie P.
McDonnell, Kevin
Donegan, Mick
Dowling, Denis P.
Permanent link: http://hdl.handle.net/10197/5269
Date: 22-Mar-2010
Abstract: Protein adhesion is of key importance for the biocompatibility of medical devices. This study investigates the adsorption of protein, bovine serum albumin (BSA), onto both uncoated silicon wafers and nanometre thick fluorosiloxane coated wafers. A plasma polymerised coating was deposited from a mixture of tetramethylcyclotetrasiloxane (TC) and perfluorooctyltriethoxysilane (FS) (1:1 by vol. ratio). The liquid precursor mixture was nebulised into an atmospheric plasma jet formed using the PlasmaStreamTM system. The adsorption of protein on the plasma polymerised coatings was evaluated under dynamic flow conditions using a spectroscopic ellipsometry technique. The rate of protein adsorption onto coated and uncoated silicon wafer substrates was monitored over time after the BSA solution was introduced into a flow cell. These measurements indicated the adsorption of a 2 nm thick BSA protein layer on the uncoated silicon wafers. The ellipsometry thickness measurements of adsorbed protein on silicon wafer were confirmed using quartz crystal microbalance measurements (QCM). The BSA adsorption studies were then repeated with a fluorosiloxane coating. These coatings exhibited a highly textured surface morphology with low surface energy and a high water contact angle of 156 . The ellipsometry flow cell tests with BSA indicated almost no adsorption of protein onto the superhydrophobic fluorosiloxane coating. This study demonstrated the ability of ellipsometry to measure protein adsorption under dynamic flow 2 conditions and the influence of surface properties on protein adsorption.
Type of material: Conference Publication
Keywords: Protein adhesion;Medical devices
Language: en
Status of Item: Peer reviewed
Conference Details: European Conference of Nanofilms (ECNF 2010), Liege, Belgium, 22 to 25 March 2010
Appears in Collections:Mechanical & Materials Engineering Research Collection

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