Evaluation of cell behaviour on atmospheric plasma deposited siloxane and fluorosiloxane coatings

Title: Evaluation of cell behaviour on atmospheric plasma deposited siloxane and fluorosiloxane coatings
Authors: Ardhaoui, Malika
Naciri, Mariam
Mullen, Tracy
Brugha, Cathal
Keenan, Alan K.
Al-Rubeai, Mohamed
Dowling, Denis P.
Permanent link: http://hdl.handle.net/10197/5270
Date: 2010
Abstract: For developing functional biomaterials, an understanding of the biological response at material surfaces is of key importance. In particular, surface chemistry, roughness and cell type influence this response. Many previous reports in the literature have involved the study of single cell types and their adhesion to surfaces with a limited range of water contact angles. The objective of this study was to investigate the adhesion of five cell lines on surfaces with contact angles in the range of 20 to 115 . This range of water contact angles was obtained using siloxane and fluorosiloxane coatings deposited using atmospheric plasma deposition. These nm thick coatings were deposited by nebulizing liquid precursors consisting of poly(dimethylsiloxane) (PDMS) and a mixture of perfluorodecyl acrylate/ tetraethylorthosilicate (PPFDA/TEOS) into the atmospheric plasmas. Cell adhesion studies were carried out with the following cell types: Osteoblast, Human Embryonic Kidney (HEK), Chinese hamster ovary (CHO), Hepatocytes (HepZ) and THP1 leukemic cells. The study demonstrated that cell adhesion was significantly influenced by the type of cell line, water contact angle and coating chemistry. For example the sensitivity of cell lines to changes in contact angle was found to decrease in the following order: Osteoblasts >Hepatocytes> CHO. The HEK and THP-1 inflammatory cells in contrast were not found to be sensitive to changes in water contact angle.
Funding Details: Science Foundation Ireland
Type of material: Journal Article
Publisher: Taylor & Francis
Copyright (published version): 2010, Koninklijke Brill NV
Keywords: Atmospheric plasma;Siloxane/ fluorosiloxane coatings;Wettability;Cell adhesion;Cell line
DOI: 10.1163/016942409X12598231567943
Language: en
Status of Item: Peer reviewed
Appears in Collections:Mechanical & Materials Engineering Research Collection

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This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. For other possible restrictions on use please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.