Isolation and Phenotypic Characterisation of Stem Cells from Late Stage Osteoarthritic Mesenchymal Tissues

Files in This Item:
File Description SizeFormat 
Labusca_CSCRT 2ND revised.pdf462.2 kBAdobe PDFDownload
Title: Isolation and Phenotypic Characterisation of Stem Cells from Late Stage Osteoarthritic Mesenchymal Tissues
Authors: Labusca, Luminita
Zugun-Eloae, Florin
Shaw, Georgina
et al.
Permanent link: http://hdl.handle.net/10197/5585
Date: 1-Aug-2012
Abstract: Introduction: Osteoarthritis (OA) represents an increasing health issue worldwide. Regenerative medicine (RM) has raised the hope for introducing revolutionary therapies in clinical practice. Detection of autologus cell sources can improve accessibility to RM strategies. Objectives: To assess the presence and biological potential of mesehchymal stem cells in three tissues (subchondral bone, synovial layer, periarticular adipose tissue) in late stages osteoarthritic patients. Material and Methods: Samples were collected from subjects undergoing total knee replacement (TKR). MSCs were isolated and cultured in complete αMEM with β FGF. Cell morphology and growth potential was assessed. Flow cytometry was used for detection of several relevant cell surface markers. Quantitative and qualitative assessment of differentiation potential towards three mesenchymal lineages (osteogenesis adipogenesis chondrogenesis) was performed. Time lapse life cell imaging of nondiferentiated cells over 24 hours period was used to determine cell kinetics. Results: Mesenchymal cells derived from all donors and tissue types showed morphology, growth and surface cell markers associated with stemness. All cell types underwent differentiation toward three mesenchymal lineages with significant differences between tissues of origin, not between donors. Cell kinetics, as derived from life imaging records, was variable with tissue of origin, significant higher for adipose derived MSCS. Conclusion: Human late stage OA mesenchymal tissues, contain progenitors with proliferative and differentiation potential of MSCs. These populations can be used for research and autologus regenerative therapies. Further comparative studies with age matched non OA samples has the potential of contributing to deepening knowledge about disease occurrence and progression.
Type of material: Journal Article
Publisher: Bentham Science
Copyright (published version): 2012 Bentham Science
Keywords: Chondrogenesis;mesenchymal stem cells;osteoarthritis;regenerative medicine;osteogenesis adipogenesis chondrogenesis;subchondral bone;synovial layer;periarticular adipose tissue;Joint recostructive surgery
DOI: 10.2174/157488812802481490
Language: en
Status of Item: Peer reviewed
Appears in Collections:Conway Institute Research Collection
SBI Research Collection

Show full item record

SCOPUSTM   
Citations 50

5
Last Week
0
Last month
checked on Jun 23, 2018

Page view(s) 50

31
checked on May 25, 2018

Download(s) 50

196
checked on May 25, 2018

Google ScholarTM

Check

Altmetric


This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. For other possible restrictions on use please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.