DMSO enhances electrospray response, boosting sensitivity of proteomic experiments

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Title: DMSO enhances electrospray response, boosting sensitivity of proteomic experiments
Authors: Hahne, Hannes
Pachl, Fiona
Ruprecht, Benjamin
Wilm, Matthias
et al.
Permanent link: http://hdl.handle.net/10197/5638
Date: Oct-2013
Abstract: We report that low percentages of dimethylsulfoxide (DMSO) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom-up proteomics by up to tenfold. The method can be easily implemented on any LC-MS/MS system without modification to hardware or software and at no additional cost.
Type of material: Journal Article
Publisher: Nature Publishing Group
Copyright (published version): 2013 Nature America
Keywords: DMSO;Proteomics;Electrospray ionization;Mass spectrometry
DOI: 10.1038/nmeth.2610
Language: en
Status of Item: Peer reviewed
Appears in Collections:Conway Institute Research Collection

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