Upon impact: the fate of adhering Pseudomonas fluorescens cells during Nanofiltration

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Title: Upon impact: the fate of adhering Pseudomonas fluorescens cells during Nanofiltration
Authors: Habimana, Olivier
Correia-Semião, Andrea Joana C.
Casey, Eoin
Permanent link: http://hdl.handle.net/10197/5888
Date: 29-Jul-2014
Online since: 2015-07-29T03:00:10Z
Abstract: Nanofiltration (NF) is a high-pressure membrane filtration process increasingly applied in drinking water treatment and water reuse processes. NF typically rejects divalent salts, organic matter, and micropollutants. However, the efficiency of NF is adversely affected by membrane biofouling, during which microorganisms adhere to the membrane and proliferate to create a biofilm. Here we show that adhered Pseudomonas fluorescens cells under high permeate flux conditions are met with high fluid shear and convective fluxes at the membrane-liquid interface, resulting in their structural damage and collapse. These results were confirmed by fluorescent staining, flow cytometry, and scanning electron microscopy. This present study offers a 'first-glimpse' of cell damage and death during the initial phases of bacterial adhesion to NF membranes and raises a key question about the role of this observed phenomena during early-stage biofilm formation under permeate flux and cross-flow conditions.
Type of material: Journal Article
Publisher: American Chemical Society
Journal: Environmental Science and Technology
Volume: 48
Issue: 16
Start page: 9641
End page: 9650
Copyright (published version): 2014 American Chemical Society
Keywords: BiofilmNanofiltration pseudomonas fluorescensCellBacterial adhesionNanofiltrationPermeate fluxHydrodynamic shearCell fate
DOI: 10.1021/es500585e
Language: en
Status of Item: Peer reviewed
Appears in Collections:Chemical and Bioprocess Engineering Research Collection

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