The significance of calcium ions on Pseudomonas fluorescens biofilms – a structural and mechanical study

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Title: The significance of calcium ions on Pseudomonas fluorescens biofilms – a structural and mechanical study
Authors: Safari, Ashkan
Habimana, Olivier
Allen, Ashley
Casey, Eoin
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Date: 13-Aug-2014
Online since: 2015-08-13T03:00:11Z
Abstract: The purpose of this study was to investigate the effects of calcium ions on the structural and mechanical properties of Pseudomonas fluorescens biofilms grown for 48 h. Advanced investigative techniques such as confocal laser scanning microscopy and atomic force spectroscopy were employed to characterize biofilm structure as well as biofilm mechanical properties following growth at different calcium concentrations. The presence of calcium during biofilm development led to higher surface coverage with distinct structural phenotypes in the form of a granular and heterogeneous surface, compared with the smoother and homogeneous biofilm surface in the absence of calcium. The presence of calcium also increased the adhesive nature of the biofilm, while reducing its elastic properties. These results suggest that calcium ions could have a functional role in biofilm development and have practical implications, for example, in analysis of biofouling in membrane-based water-treatment processes such as nanofiltration or reverse osmosis where elevated calcium concentrations may occur at the solid–liquid interface.
Type of material: Journal Article
Publisher: Taylor & Francis
Journal: Biofouling: The Journal of Bioadhesion and Biofilm Research
Volume: 30
Issue: 7
Start page: 859
End page: 869
Copyright (published version): 2014 Taylor and Francis
Keywords: Pseudomonas fluorescensBiofilmCaCl2Force spectroscopyYoung’s modulus
DOI: 10.1080/08927014.2014.938648
Language: en
Status of Item: Peer reviewed
Appears in Collections:Chemical and Bioprocess Engineering Research Collection

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