Characterisitics of Streptomyces griseus biofilms in continuous flow tubular reactors

Title: Characterisitics of Streptomyces griseus biofilms in continuous flow tubular reactors
Authors: Winn, Michael
Habimana, Olivier
Casey, Eoin
Murphy, Cormac D.
Permanent link: http://hdl.handle.net/10197/6341
Date: Mar-2014
Online since: 2015-02-05T12:27:40Z
Abstract: The purpose of this study was to investigate the feasibility of cultivating the biotechnologically important bacterium Streptomyces griseus in single-species and mixed- species biofilms using a Tubular Biofilm Reactor (TBR). Streptomyces griseus biofilm development was found to be cyclical, starting with the initial adhesion and subsequent development of a visible biofilm after 24 hours growth, followed by the complete detachment of the biofilm as a single mass, and ending with the re-colonization of the tube. Fluorescence microscopy revealed that the filamentous structure of the biofilm was lost upon treatment with protease, but not DNase or metaperiodate, indicating that the extracellular polymeric substance is predominantly protein. When the biofilm was cultivated in conjunction with Bacillus amyloliquefaciens, no detachment was observed after 96 h, although once subjected to flow detachment occurred. Electron microscopy confirmed the presence of both bacteria in the biofilm and revealed a network of fimbriae-like structures that were much less apparent in single-species biofilm, and are likely to increase mechanical stability when developing in a TBR. This study presents the very first attempt in engineering Streptomyces griseus biofilms for continuous bioprocess applications.
Funding Details: Science Foundation Ireland
Type of material: Journal Article
Publisher: Wiley
Journal: FEMS Microbiology Letters
Volume: 352
Issue: 2
Start page: 157
End page: 164
Copyright (published version): 2014 Federation of European Microbiological Societies
Keywords: Attached growthExtracellular polymeric substancesContinuous bioprocess
DOI: 10.1111/1574-6968.12378
Language: en
Status of Item: Peer reviewed
Appears in Collections:Chemical and Bioprocess Engineering Research Collection
Biomolecular and Biomedical Science Research Collection

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