Characterisitics of Streptomyces griseus biofilms in continuous flow tubular reactors

DC FieldValueLanguage
dc.contributor.authorWinn, Michael-
dc.contributor.authorHabimana, Olivier-
dc.contributor.authorCasey, Eoin-
dc.contributor.authorMurphy, Cormac D.-
dc.date.accessioned2015-02-05T12:27:40Z-
dc.date.available2015-02-05T12:27:40Z-
dc.date.copyright2014 Federation of European Microbiological Societiesen
dc.date.issued2014-03-
dc.identifier.citationFEMS Microbiology Lettersen
dc.identifier.urihttp://hdl.handle.net/10197/6341-
dc.description.abstractThe purpose of this study was to investigate the feasibility of cultivating the biotechnologically important bacterium Streptomyces griseus in single-species and mixed- species biofilms using a Tubular Biofilm Reactor (TBR). Streptomyces griseus biofilm development was found to be cyclical, starting with the initial adhesion and subsequent development of a visible biofilm after 24 hours growth, followed by the complete detachment of the biofilm as a single mass, and ending with the re-colonization of the tube. Fluorescence microscopy revealed that the filamentous structure of the biofilm was lost upon treatment with protease, but not DNase or metaperiodate, indicating that the extracellular polymeric substance is predominantly protein. When the biofilm was cultivated in conjunction with Bacillus amyloliquefaciens, no detachment was observed after 96 h, although once subjected to flow detachment occurred. Electron microscopy confirmed the presence of both bacteria in the biofilm and revealed a network of fimbriae-like structures that were much less apparent in single-species biofilm, and are likely to increase mechanical stability when developing in a TBR. This study presents the very first attempt in engineering Streptomyces griseus biofilms for continuous bioprocess applications.en
dc.description.sponsorshipScience Foundation Irelanden
dc.language.isoenen
dc.publisherWileyen
dc.rightsThis is the author's version of the following article: Michael Winn, Eoin Casey, Olivier Habimana and Cormac D. Murphy (2014) "FEMS Microbiology Letters", 352(2) : 157-164 which has been published in final form at http://dx.doi.org/10.1111/1574-6968.12378en
dc.subjectAttached growthen
dc.subjectExtracellular polymeric substancesen
dc.subjectContinuous bioprocessen
dc.titleCharacterisitics of Streptomyces griseus biofilms in continuous flow tubular reactorsen
dc.title.alternativeStreptomyces griseus biofilmen
dc.typeJournal Articleen
dc.internal.authorcontactothercormac.d.murphy@ucd.ie-
dc.internal.availabilityFull text availableen
dc.statusPeer revieweden
dc.identifier.volume352en
dc.identifier.issue2en
dc.identifier.startpage157en
dc.identifier.endpage164en
dc.identifier.doi10.1111/1574-6968.12378-
dc.neeo.contributorWinn|Michael|aut|-
dc.neeo.contributorHabimana|Olivier|aut|-
dc.neeo.contributorCasey|Eoin|aut|-
dc.neeo.contributorMurphy|Cormac D.|aut|-
dc.internal.rmsid378480552-
dc.date.updated2015-02-02T09:35:23Z-
item.grantfulltextopen-
item.fulltextWith Fulltext-
Appears in Collections:Chemical and Bioprocess Engineering Research Collection
Biomolecular and Biomedical Science Research Collection
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